2026-05-22T03:12:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/268142025-12-18T13:01:53Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Rigoni, Giovanni
author
Calvo, Enrique
author
Glytsou, Christina
author
Carro-Alvarellos, Marta
author
Noguchi, Masafumi
author
Semenzato, Martina
author
Quirin, Charlotte
author
Caicci, Federico
author
Meneghetti, Natascia
author
Sturlese, Mattia
author
Ishihara, Takaya
author
Moro, Stefano
author
Rampazzo, Chiara
author
Ishihara, Naotada
author
Bezzo, Fabrizio
author
Salviati, Leonardo
author
Vazquez, Jesùs
author
Sales, Gabriele
author
Romualdi, Chiara
author
Enriquez, Jose Antonio
author
Scorrano, Luca
author
Soriano, Maria Eugenia
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Fundación La Caixa
2025-07-09T13:57:47Z
2025-07-09T13:57:47Z
2025-04-01
Cell Metab. 2025 Apr 1;37(4):1024-1038.e8.
Cell Metabolism
39999845
https://hdl.handle.net/20.500.12105/26814
Mitochondrial proteins assemble dynamically in high molecular weight complexes essential for their functions. We generated and validated two searchable compendia of these mitochondrial complexes. Following identification by mass spectrometry of proteins in complexes separated using blue-native gel electrophoresis from unperturbed, cristae-remodeled, and outer membrane-permeabilized mitochondria, we created MARIGOLD, a mitochondrial apoptotic remodeling complexome database of 627 proteins. MARIGOLD elucidates how dynamically proteins distribute in complexes upon mitochondrial membrane remodeling. From MARIGOLD, we developed MitoCIAO, a mitochondrial complexes interactome tool that, by statistical correlation, calculates the likelihood of protein cooccurrence in complexes. MitoCIAO correctly predicted biologically validated interactions among components of the mitochondrial cristae organization system (MICOS) and optic atrophy 1 (OPA1) complexes. We used MitoCIAO to functionalize two ATPase family AAA domain-containing 3A (ATAD3A) complexes: one with OPA1 that regulates mitochondrial ultrastructure and the second containing ribosomal proteins that is essential for mitoribosome stability. These compendia reveal the dynamic nature of mitochondrial complexes and enable their functionalization.
eng
ATAD3A
OPA1
cristae remodeling
interactome
mitochondria
mitochondrial complexes
mitoribosome stability
MARIGOLD and MitoCIAO, two searchable compendia to visualize and functionalize protein complexes during mitochondrial remodeling.
research article
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URL
https://repisalud.isciii.es/bitstreams/fd0f4890-1a3c-4a6e-9553-29121c8dbf88/download
File
MD5
5b1291feb0bd0225885165efab905dc8
8544915
application/pdf
MARIGOLD and MitoCIAO_Cell Metab_2025.pdf