2026-05-22T00:57:25Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/268142025-12-18T13:01:53Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Rigoni, Giovanni author Calvo, Enrique author Glytsou, Christina author Carro-Alvarellos, Marta author Noguchi, Masafumi author Semenzato, Martina author Quirin, Charlotte author Caicci, Federico author Meneghetti, Natascia author Sturlese, Mattia author Ishihara, Takaya author Moro, Stefano author Rampazzo, Chiara author Ishihara, Naotada author Bezzo, Fabrizio author Salviati, Leonardo author Vazquez, Jesùs author Sales, Gabriele author Romualdi, Chiara author Enriquez, Jose Antonio author Scorrano, Luca author Soriano, Maria Eugenia author 2025-04-01 Mitochondrial proteins assemble dynamically in high molecular weight complexes essential for their functions. We generated and validated two searchable compendia of these mitochondrial complexes. Following identification by mass spectrometry of proteins in complexes separated using blue-native gel electrophoresis from unperturbed, cristae-remodeled, and outer membrane-permeabilized mitochondria, we created MARIGOLD, a mitochondrial apoptotic remodeling complexome database of 627 proteins. MARIGOLD elucidates how dynamically proteins distribute in complexes upon mitochondrial membrane remodeling. From MARIGOLD, we developed MitoCIAO, a mitochondrial complexes interactome tool that, by statistical correlation, calculates the likelihood of protein cooccurrence in complexes. MitoCIAO correctly predicted biologically validated interactions among components of the mitochondrial cristae organization system (MICOS) and optic atrophy 1 (OPA1) complexes. We used MitoCIAO to functionalize two ATPase family AAA domain-containing 3A (ATAD3A) complexes: one with OPA1 that regulates mitochondrial ultrastructure and the second containing ribosomal proteins that is essential for mitoribosome stability. These compendia reveal the dynamic nature of mitochondrial complexes and enable their functionalization. Cell Metab. 2025 Apr 1;37(4):1024-1038.e8. Cell Metabolism 39999845 https://hdl.handle.net/20.500.12105/26814 ATAD3A OPA1 cristae remodeling interactome mitochondria mitochondrial complexes mitoribosome stability MARIGOLD and MitoCIAO, two searchable compendia to visualize and functionalize protein complexes during mitochondrial remodeling.