2026-07-21T07:57:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/266772025-12-18T12:58:33Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Santin, Monica
author
Figueiredo, Ana
author
Molokin, Aleksey
author
Carmena, David
author
George, Nadja S
author
Köster, Pamela Carolina
author
Dashti, Alejandro
author
Gonzalez-Barrio, David
author
Carmena, David
author
Maloney, Jenny G
funder
United States Department of Agriculture
funder
Fundação para a Ciência e Tecnologia (Portugal)
funder
Unión Europea. Fondo Social Europeo (ESF/FSE)
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Unión Europea. European Cooperation in Science and Technology (COST)
2025-06-04T13:51:46Z
2025-06-04T13:51:46Z
2024
Santin M, Figueiredo A, Molokin A, George NS, Köster PC, Dashti A, González-Barrio D, Carmena D, Maloney JG. Division of Blastocystis ST10 into three new subtypes: ST42-ST44. J Eukaryot Microbiol. 2024 Jan-Feb;71(1):e12998.
10.1111/jeu.12998
1550-7408
1066-5234
The Journal of eukaryotic microbiology
37658622
https://hdl.handle.net/20.500.12105/26677
The Blastocystis subtype ST10 has been recognized to contain a great deal of diversity at the sequence level, potentially indicating the presence of multiple new STs within the clade. However, the data needed to validate these new STs were not available. To help resolve this diversity, full-length small subunit (SSU) rRNA gene reference sequences were generated using Oxford Nanopore MinION long-read sequencing from 21 samples representing multiple domestic and wild hosts and geographic regions and covering the sequence diversity previously described using fragments of the SSU rRNA gene. Phylogenetic and pairwise distance analyses were used to compare full-length sequences of the SSU rRNA gene generated in this study with all other valid STs of Blastocystis. We present data supporting the division of ST10/ST23 cluster into five subtypes, ST10, ST23, and three new subtypes with the proposed ST designations of ST42, ST43, and ST44. As the host range of Blastocystis continues to expand with new subtypes and new hosts being frequently identified, the reference sequences provided in this study will assist in accurate sequence classification and help to clarify the epidemiology of this common intestinal microeukaryote.
eng
Blastocystis
MinION
ST10
ST23
ST42
ST43
ST44
Small subunit ribosomal RNA gene
Subtype
Division of Blastocystis ST10 into three new subtypes: ST42-ST44
research article
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DivisionBlastocystis_ST10_2024.pdf
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Supplementary_DivisionBlastocystis_ST10_2024.pdf