2026-07-22T03:37:21Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/265812025-12-18T13:00:08Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Casadémont, Isabelle
author
Ayala-Suarez, Ruben
author
Modhiran, Naphak
author
Tawfik, Ahmed
author
Prot, Matthieu
author
Paul, Richard
author
Simon-Lorière, Etienne
author
Díez-Fuertes, Francisco
author
Ubol, Sukathida
author
Alcamí, José
author
Sakuntabhai, Anavaj
funder
Unión Europea. Comisión Europea. 7 Programa Marco
funder
Agence Nationale de la Recherche (Francia)
funder
Institut Pasteur
funder
NIH - National Institute of Allergy and Infectious Diseases (NIAID) (Estados Unidos)
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia (España)
2025-03-27T11:07:36Z
2025-03-27T11:07:36Z
2025-02-20
Casadémont I, Ayala-Suárez R, Modhiran N, Tawfik A, Prot M, Paul R, Simon-Lorière E, Díez-Fuertes F, Ubol S, Alcamí J, Sakuntabhai A. miRNome analysis reveals mir-155-5p as a protective factor to dengue infection in a resistant Thai cohort. Med Microbiol Immunol. 2025 Feb 20;214(1):13.
10.1007/s00430-025-00821-7
1432-1831
0300-8584
Medical microbiology and immunology
39976655
https://hdl.handle.net/20.500.12105/26581
Dengue virus (DENV) is a global health threat, with approximately 390 million infections annually, ranging from mild dengue fever to severe dengue hemorrhagic fever and shock syndrome. MicroRNA (miRNA) are crucial post-transcriptional regulators which may regulate host resistance to DENV infection. This study aimed to identify miRNAs involved in natural resistance to DENV infection. Individuals from a dengue-endemic area were classified as susceptible (SD) or resistant (RD) according to their anti-DENV antibody status. RD individuals were seronegative despite high local DENV infection prevalence. Monocytes susceptibility to DENV infection was assessed in vitro. The miRNome profiles of the monocytes from 7 individuals per group were assessed upon mock or DENV-2 infection. The antiviral effect of differentially expressed miRNAs was analyzed using miRNA mimics in HeLa cells followed by infection with DENV-1, DENV-2, DENV-3, and DENV-4 serotypes. We performed RNA-seq on miRNA mimic-transfected cells to identify miRNA-targeted genes interacting with DENV proteins. Monocytes from RD individuals exhibit lower DENV-2 production in vitro. The miRNAs miR-155, miR-132-3p, miR-576-5p were overexpressed in monocytes from RD group upon DENV-2 infection. The transfection of miR-155-5p mimic reduced DENV infection and viral production in HeLa cells, regulating 18 genes interacting with DENV proteins and downregulating target genes involved in interferon response, TP53 regulation, apoptosis, and vesicle trafficking (e.g. HSD17B12, ANXA2). Therefore, we show that monocytes from RD individuals show a distinct miRNA expression profile and reduced viral production. In vitro miR-155-5p upregulation induces an antiviral state, revealing potential therapeutic targets to treat dengue.
eng
Dengue
RNA-Seq
Resistance
miR-155-5p
miRNome
microRNA
miRNome analysis reveals mir-155-5p as a protective factor to dengue infection in a resistant Thai cohort
research article
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miR_NomeAnalysisReveals_mir-155-5p_2025.pdf
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Supplementary_miRNome analysis reveals mir-155-5p_2025.7z