2026-04-26T23:12:51Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/265782025-12-18T12:59:37Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Zaragoza, Gloria
author
Perez-Vazquez, Maria
author
Villar-Gómara, Laura
author
González-Prieto, Andrea
author
Oteo-Iglesias, Jesus
author
Alós, Juan-Ignacio
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Comisión Europea. NextGenerationEU
2025-03-26T13:57:16Z
2025-03-26T13:57:16Z
2024-02-27
Zaragoza G, Pérez-Vázquez M, Villar-Gómara L, González-Prieto A, Oteo-Iglesias J, Alós JI. Community Emergence of Cefixime-Resistant Escherichia coli Belonging to ST12 with Chromosomal AmpC Hyperproduction. Antibiotics (Basel). 2024 Feb 27;13(3):218.
https://hdl.handle.net/20.500.12105/26578
38534653
10.3390/antibiotics13030218
2079-6382
Antibiotics (Basel, Switzerland)
Escherichia coli isolates that are resistant to cefixime and amoxicillin/clavulanic acid, but apparently susceptible to cefuroxime, with no ESBL identified, were initially detected in Madrid from urine samples in 2019. Throughout 2020 and 2021, all cases of community UTI by E. coli from six health areas in Madrid were studied. A representative sample of 23 cases was selected for further studies. The broth microdilution method and the agar diffusion method were performed to determine the antibiotic susceptibility. WGS was carried out for phylogeny, resistome and virulome analysis. Community consumption of third-generation oral cephalosporins in Madrid (2017-2021) was analyzed. A total of 582 (1.3%) E. coli isolates had the mentioned resistance profile. The mutation at position -32 (T > A) of the AmpC promoter was found in 21 isolates. No plasmid AmpC- or ESBL-encoding genes were detected. A cluster of 20 ST12 isolates was detected by cgMLST. A 6.2% increase in the consumption of third-generation oral cephalosporins, especially cefixime, was observed in Madrid. Chromosomal AmpC-hyperproducing ST12 E. coli isolates could be implicated in the increase in community UTI cases by cefixime-resistant isolates, which correlates with an increasing trend of cefixime consumption.
eng
AmpC
Escherichia coli
ST12
Cefixime
Community Emergence of Cefixime-Resistant Escherichia coli Belonging to ST12 with Chromosomal AmpC Hyperproduction
research article
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URL
https://repisalud.isciii.es/bitstreams/351731f1-7483-4fa4-a3c6-d629e9370946/download
File
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062e0ed41be9328e6c37ed4f39d45ca1
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application/pdf
CommunityEmergenceCefixime-ResistantEscherichia_coli_2024.pdf