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oai:repisalud.isciii.es:20.500.12105/265782025-12-18T12:59:37Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Zaragoza, Gloria author Perez-Vazquez, Maria author Villar-Gómara, Laura author González-Prieto, Andrea author Oteo-Iglesias, Jesus author Alós, Juan-Ignacio author 2024-02-27 Escherichia coli isolates that are resistant to cefixime and amoxicillin/clavulanic acid, but apparently susceptible to cefuroxime, with no ESBL identified, were initially detected in Madrid from urine samples in 2019. Throughout 2020 and 2021, all cases of community UTI by E. coli from six health areas in Madrid were studied. A representative sample of 23 cases was selected for further studies. The broth microdilution method and the agar diffusion method were performed to determine the antibiotic susceptibility. WGS was carried out for phylogeny, resistome and virulome analysis. Community consumption of third-generation oral cephalosporins in Madrid (2017-2021) was analyzed. A total of 582 (1.3%) E. coli isolates had the mentioned resistance profile. The mutation at position -32 (T > A) of the AmpC promoter was found in 21 isolates. No plasmid AmpC- or ESBL-encoding genes were detected. A cluster of 20 ST12 isolates was detected by cgMLST. A 6.2% increase in the consumption of third-generation oral cephalosporins, especially cefixime, was observed in Madrid. Chromosomal AmpC-hyperproducing ST12 E. coli isolates could be implicated in the increase in community UTI cases by cefixime-resistant isolates, which correlates with an increasing trend of cefixime consumption. Zaragoza G, Pérez-Vázquez M, Villar-Gómara L, González-Prieto A, Oteo-Iglesias J, Alós JI. Community Emergence of Cefixime-Resistant Escherichia coli Belonging to ST12 with Chromosomal AmpC Hyperproduction. Antibiotics (Basel). 2024 Feb 27;13(3):218. https://hdl.handle.net/20.500.12105/26578 38534653 10.3390/antibiotics13030218 2079-6382 Antibiotics (Basel, Switzerland) AmpC Escherichia coli ST12 Cefixime Community Emergence of Cefixime-Resistant Escherichia coli Belonging to ST12 with Chromosomal AmpC Hyperproduction