2026-04-29T01:00:11Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/265432025-12-18T13:00:02Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Casais, Rosa
author
Iglesias, Natalia
author
Sevilla, Iker A
author
Garrido, Joseba M
author
Balseiro, Ana
author
Dominguez-Rodriguez, Mercedes
author
Juste, Ramón A
funder
Gobierno del Principado de Asturias (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2025-03-20T13:08:53Z
2025-03-20T13:08:53Z
2024-03-26
Casais R, Iglesias N, Sevilla IA, Garrido JM, Balseiro A, Dominguez M, Juste RA. Non-specific effects of inactivated Mycobacterium bovis oral and parenteral treatment in a rabbit scabies model. Vet Res. 2024 Mar 26;55(1):41.
0928-4249
https://hdl.handle.net/20.500.12105/26543
38532491
10.1186/s13567-024-01293-y
1297-9716
Veterinary research
Tuberculosis BCG vaccination induced non-specific protective effects in humans led to postulate the concept of trained immunity (TRAIM) as an innate type of immune mechanism that triggered by a pathogen, protects against others. Killed vaccines have been considered not to be effective. However, field efficacy of a commercial vaccine against paratuberculosis, as well as of a recently developed M. bovis heat-inactivated vaccine (HIMB) prompted to test whether it could also induce TRAIM. To this, we used a sarcoptic mange rabbit model. Twenty-four weaned rabbits were treated orally or subcutaneously with a suspension of either HIMB (107 UFC) or placebo. Eighty-four days later the animals were challenged with approximately 5000 S. scabiei mites on the left hind limb. Skin lesion extension was measured every 2 weeks until 92 days post-infection (dpi). Two animals were killed at 77 dpi because of extensive skin damage. The rest were euthanized and necropsied and the lesion area and the mite burden per squared cm were estimated. Specific humoral immune responses to S. scabiei and to M. bovis were investigated with the corresponding specific ELISA tests. Subcutaneously and orally HIMB vaccinated animals compared with placebo showed reduced lesion scores (up to 74% and 62%, respectively) and mite counts (-170% and 39%, respectively). This, together with a significant positive correlation (r = 0.6276, p = 0.0031) between tuberculosis-specific antibodies and mite count at 92 dpi supported the hypothesis of non-specific effects of killed mycobacterial vaccination. Further research is needed to better understand this mechanism to maximize cross protection.
eng
Mycobacterium bovis
Sarcoptes scabiei
Killed vaccine
Scabies
Serology
Trained immunity
Tuberculosis
Non-specific effects of inactivated Mycobacterium bovis oral and parenteral treatment in a rabbit scabies model
research article
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URL
https://repisalud.isciii.es/bitstreams/08b02bc7-c348-4a22-9765-9ad334160fb9/download
File
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fdb38cc43521b8fc8a6e6e37a7777e37
2144156
application/pdf
Non-specificEffectsInactivatedMycobacteriumBovis_2024.pdf