2026-07-21T20:18:23Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/265162025-12-18T13:00:41Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16958col_20.500.12105_16985

00925njm 22002777a 4500 dc Pacios, Olga author Herrera-Espejo, Soraya author Armán, Lucía author Ibarguren-Quiles, Clara author Blasco, Lucía author Bleriot, Inés author Fernández-García, Laura author Ortiz-Cartagena, Concha author Paniagua, María author Barrio-Pujante, Antonio author Aracil, Belen author Cisneros, José Miguel author Pachón-Ibáñez, María Eugenia author Tomás, María author 2024-08-28 The combination of several therapeutic strategies is often seen as a good way to decrease resistance rates, since bacteria can more easily overcome single-drug treatments than multi-drug ones. This strategy is especially attractive when several targets and subpopulations are affected, as it is the case of Klebsiella pneumoniae persister cells, a subpopulation of bacteria able to transiently survive antibiotic exposures. This work aims to evaluate the potential of a repurposed anticancer drug, mitomycin C, combined with the K. pneumoniae lytic phage vB_KpnM-VAC13 in vitro and its safety in an in vivo murine model against two clinical isolates of this pathogen, one of them exhibiting an imipenem-persister phenotype. At the same time, we verified the absence of toxicity of mitomycin C at the concentration using the human chondrocyte cell line T/C28a2. The viability of these human cells was checked using both cytotoxicity assays and flow cytometry. Pacios O, Herrera-Espejo S, Armán L, Ibarguren-Quiles C, Blasco L, Bleriot I, Fernández-García L, Ortiz-Cartagena C, Paniagua M, Barrio-Pujante A, Aracil B, Cisneros JM, Pachón-Ibáñez ME, Tomás M. Mitomycin C as an Anti-Persister Strategy against Klebsiella pneumoniae: Toxicity and Synergy Studies. Antibiotics (Basel). 2024 Aug 28;13(9):815. 10.3390/antibiotics13090815 2079-6382 Antibiotics (Basel, Switzerland) 39334989 https://hdl.handle.net/20.500.12105/26516 K. pneumoniae Cytotoxicity Lyticphage Mitomycin C Persistence Repurposing Mitomycin C as an Anti-Persister Strategy against Klebsiella pneumoniae: Toxicity and Synergy Studies