2026-05-22T08:52:31Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/264922025-12-18T13:00:11Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16963col_20.500.12105_16964
Repisalud
author
Cabrera-Rubio, Raul
author
Calvo, Cristina
author
Alcolea, Sonia
author
Bergia, María
author
Atucha, Jorge
author
Pozo Sanchez, Francisco
author
Casas Flecha, Inmaculada
author
Arroyas, María
author
Collado, Maria Carmen
author
García-García, María Luz
funder
Fundación Universidad Alfonso X el Sabio
funder
Merck, Sharp & Dohme
funder
Instituto de Salud Carlos III
funder
Generalitat Valenciana (España)
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Agencia Estatal de Investigación (España)
2025-03-17T10:39:04Z
2025-03-17T10:39:04Z
2024-07-02
Cabrera-Rubio R, Calvo C, Alcolea S, Bergia M, Atucha J, Pozo F, Casas I, Arroyas M, Collado MC, García-García ML. Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome? Microbiol Spectr. 2024 Jul 2;12(7):e0255623.
10.1128/spectrum.02556-23
2165-0497
Microbiology spectrum
38785596
https://hdl.handle.net/20.500.12105/26492
Growing evidence indicates that gut and respiratory microbiota have a potential key effect on bronchiolitis, mainly caused by respiratory syncytial virus (RSV). This was a prospective study of 96 infants comparing infants with bronchiolitis (n = 57, both RSV and non-RSV associated) to a control group (n = 39). Gut (feces) and respiratory [nasopharyngeal aspirate (NPA)] microbial profiles were analyzed by 16S rRNA amplicon sequencing, and respiratory viruses were identified by PCR. Clinical data of the acute episode and follow-up during the first year after infection were recorded. Pairwise comparisons showed significant differences in the gut (R2 = 0.0639, P = 0.006) and NPA (R2 = 0.0803, P = 0.006) microbiota between cases and controls. A significantly lower gut microbial richness and an increase in the NPA microbial diversity (mainly due to an increase in Haemophilus, Streptococcus, and Neisseria) were observed in the infants with bronchiolitis, in those with the most severe symptoms, and in those who subsequently developed recurrent wheezing episodes after discharge. In NPA, the higher microbial richness differed significantly between the control group and the non-RSV bronchiolitis group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001). In the gut, the richness differed significantly between the control group and the non-RSV group (P = 0.01) and between the control group and the RSV bronchiolitis group (P = 0.001), with higher diversity in the RSV group. A distinct respiratory and intestinal microbial pattern was observed in infants with bronchiolitis compared with controls. The presence of RSV was a main factor for dysbiosis. Lower gut microbial richness and increased respiratory microbial diversity were associated with respiratory morbidity during follow-up. Importance: Both the intestinal and respiratory microbiota of children with bronchiolitis, especially those with respiratory syncytial virus infection, are altered and differ from that of healthy children. The microbiota pattern in the acute episode could identify those children who will later have other respiratory episodes in the first year of life. Preventive measures could be adopted for this group of infants.
eng
Gut microbiota
Infants
Nasopharyngeal microbiota
Respiratory syncytial virus
Virus
Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome?
research article
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