2026-05-20T04:01:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/264392025-12-18T13:00:37Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16988
Repisalud
author
Maruri-Aransolo, Ainhize
author
de Dios Caballero, Juan
author
Michelena, Malkoa
author
Medina-Pascual, Maria Jose
author
Carrasco, Gema
author
Asensio, Oscar
author
Cols, Maria
author
Cantón, Rafael
author
GEIFQ group
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Plan Nacional de I+D+i (España)
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
2025-03-13T09:16:39Z
2025-03-13T09:16:39Z
2024-07
Maruri-Aransolo A, de Dios Caballero J, Michelena M, Medina-Pascual MJ, Carrasco G, Asensio O, Cols M, Cantón R; GEIFQ group. Evaluation of CHROMagar™ B. cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis. Eur J Clin Microbiol Infect Dis. 2024 Jul;43(7):1349-1353.
10.1007/s10096-024-04845-4
1435-4373
0934-9723
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
38780755
https://hdl.handle.net/20.500.12105/26439
Introduction: Burkholderia cepacia complex (BCC) are non-fermenting Gram-negative bacteria that can chronically colonize the lungs of people with cystic fibrosis (pwCF), causing a severe and progressive respiratory failure, post-transplant complications and epidemic outbreaks. Therefore, rapid and accurate identification of these bacteria is relevant for pwCF, in order to facilitate early eradication and prevent chronic colonization. However, BCCs are often quite difficult to detect on culture media as they have a slow growth rate and can be hidden by other fast-growing microorganisms, including Pseudomonas aeruginosa and filamentous fungi. Material and methods: We evaluated the sensitivity of CHROMagar™ B. cepacia agar using 11 isolates from a well-characterized BCC collection, using BCA agar (Oxoid, UK) as a gold standard. We also studied 180 clinical sputum samples to calculate positive (PPV) and negative (NPV) predictive values. Furthermore, we used three of the well-characterized BCC isolates to determine the limit of detection (LOD). Results: Eleven isolates grew on CHROMagar™ B. cepacia at 37ºC after 48 h. The NPV and PPV of CHROMagar™ B. cepacia were 100% and 87.5%, respectively. The LOD of CHROMagar™ B. cepacia was around 1 × 103 CFU/ml, requiring a ten-fold dilution lower bacterial load than BCA for BCC detection. Conclusion: CHROMagar™ B. cepacia agar proved to have a very good sensitivity and specificity for the detection of clinical BCCs. Moreover, the chromogenic nature of the medium allowed us to clearly differentiate BCC from other Gram-negative species, filamentous fungi and yeasts, thereby facilitating the identification of contaminants.
eng
Burkholderia cepacia complex
CHROMagar™
Chronic respiratory infections
Cystic fibrosis
Evaluation of CHROMagar™ B. cepacia agar for the detection of Burkholderia cepacia complex species from sputum samples of patients with cystic fibrosis
research article
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Evaluation_CHROMagar_B_Cepacia_2024.pdf
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Supplementary_Evaluation_CHROMagar_B_Cepacia_2024.pdf