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oai:repisalud.isciii.es:20.500.12105/264322025-12-18T13:00:38Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Witteveen, Sandra author Hans, Jörg B author Izdebski, Radosław author Hasman, Henrik author Samuelsen, Ørjan author Dortet, Laurent author Pfeifer, Yvonne author Delappe, Niall author Oteo-Iglesias, Jesus author Żabicka, Dorota author Cormican, Martin author Sandfort, Mirco author Reichert, Felix author Pöntinen, Anna K author Fischer, Martin A author Verkaik, Nelianne author Perez-Vazquez, Maria author Pfennigwerth, Niels author Hammerum, Anette M author Hallstrøm, Søren author Biedrzycka, Marta author Räisänen, Kati author Wielders, Cornelia Ch author Urbanowicz, Paweł author de Haan, Angela author Westmo, Karin author Landman, Fabian author van der Heide, Han Gj author Lansu, Simon author Zwittink, Romy D author Notermans, Daan W author Guzek, Aneta author Kondratiuk, Viacheslav author Salmanov, Aidyn author Haller, Sebastian author Linkevicius, Marius author Gatermann, Sören author Kohlenberg, Anke author Gniadkowski, Marek author Werner, Guido author Hendrickx, Antoni PA author 2024-06 Background: The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related to Ukrainian patients. Aim: To investigate the genomic epidemiology of New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from Ukrainian patients among European countries. Methods: Whole-genome sequencing of 66 isolates sampled in 2022-2023 in 10 European countries enabled whole-genome multilocus sequence typing (wgMLST), identification of resistance genes, replicons, and plasmid reconstructions. Five bla NDM-1-carrying-P. stuartii isolates underwent antimicrobial susceptibility testing (AST). Transferability to Escherichia coli of a bla NDM-1-carrying plasmid from a patient strain was assessed. Epidemiological characteristics of patients with NDM-producing P. stuartii were gathered by questionnaire. Results; wgMLST of the 66 isolates revealed two genetic clusters unrelated to Ukraine and three linked to Ukrainian patients. Of these three, two comprised bla NDM-1-carrying-P. stuartii and the third bla NDM-5-carrying-P. stuartii. The bla NDM-1 clusters (PstCluster-001, n = 22 isolates; PstCluster-002, n = 8 isolates) comprised strains from seven and four countries, respectively. The bla NDM-5 cluster (PstCluster-003) included 13 isolates from six countries. PstCluster-001 and PstCluster-002 isolates carried an MDR plasmid harbouring bla NDM-1, bla OXA-10, bla CMY-16, rmtC and armA, which was transferrable in vitro and, for some Ukrainian patients, shared by other Enterobacterales. AST revealed PstCluster-001 isolates to be extensively drug-resistant (XDR), but susceptible to cefiderocol and aztreonam-avibactam. Patients with data on age (n = 41) were 19-74 years old; of 49 with information on sex, 38 were male. Conclusion: XDR P. stuartii were introduced into European countries, requiring increased awareness and precautions when treating patients from conflict-affected areas. Witteveen S, Hans JB, Izdebski R, Hasman H, Samuelsen Ø, Dortet L, Pfeifer Y, Delappe N, Oteo-Iglesias J, Żabicka D, Cormican M, Sandfort M, Reichert F, Pöntinen AK, Fischer MA, Verkaik N, Pérez-Vazquez M, Pfennigwerth N, Hammerum AM, Hallstrøm S, Biedrzycka M, Räisänen K, Wielders CC, Urbanowicz P, de Haan A, Westmo K, Landman F, van der Heide HG, Lansu S, Zwittink RD, Notermans DW, Guzek A, Kondratiuk V, Salmanov A, Haller S, Linkevicius M, Gatermann S, Kohlenberg A, Gniadkowski M, Werner G, Hendrickx AP. Dissemination of extensively drug-resistant NDM-producing Providencia stuartii in Europe linked to patients transferred from Ukraine, March 2022 to March 2023. Euro Surveill. 2024 Jun;29(23):2300616. 1025-496X https://hdl.handle.net/20.500.12105/26432 38847120 10.2807/1560-7917.ES.2024.29.23.2300616 1560-7917 Euro surveillance : bulletin Européen sur les maladies transmissibles = European communicable disease bulletin New Delhi metallo-β-lactamase Providencia stuartii Ukrainian patients Dissemination War Dissemination of extensively drug-resistant NDM-producing in Europe linked to patients transferred from Ukraine, March 2022 to March 2023.