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oai:repisalud.isciii.es:20.500.12105/264312025-12-18T12:55:46Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16980col_20.500.12105_16978

00925njm 22002777a 4500 dc López-Suárez, Andrea author Santos-Sebastián, Mar author Hernanz-Lobo, Alicia author Rincón-López, Elena author Aguilera-Alonso, David author Saavedra-Lozano, Jesús author Ruiz Serrano, María Jesús author Hernández-Bartolomé, Ángel author Medrano, Luz Maria author Jiménez Fuentes, José Luis author Navarro, María Luisa author Tebruegge, Marc author Santiago-García, Begoña author 2024-12 Introduction: Immune-based diagnostic tests for tuberculosis (TB) have suboptimal sensitivity in children and cannot differentiate between latent infection (LTBI) and active disease. This study evaluated the diagnostic potential of a broad range of biomarkers of tissue damage and inflammation in unstimulated plasma in children. Methods: We analyzed 17 biomarkers in 15 non-M. tuberculosis (MTB)-infected controls and 33 children with TB infection (LTBI, n = 8; probable TB, n = 19; confirmed TB, n = 6). Biomarker concentrations were measured using a Luminex magnetic bead-based platform and multiplex sandwich immunoassays. Concentrations, correlations and diagnostic accuracy assessments were conducted among patient groups. Results: Confirmed TB cases had significantly higher concentrations of IFN-γ and IL-2 and higher IFN-γ/MCP-1 and IL-2/MCP-1 ratios compared to LTBI and non-MTB-infected children. Among children with confirmed TB, there was a strong correlation between IFN-γ and IL-10 (r = 0.95; p < 0.001) and a significant correlation between IL-2 and IL-1ra (r = 0.92), IL-21 (r = 0.91), MCP-3 (r = 0.84), and MMP-1 (r = 0.85). The IFN-γ/MCP-1 ratio was the most accurate biomarker combination for differentiating between MTB-infected and non-MTB-infected children (AUC, 0.82; sensitivity, 87.9%; specificity, 66.6%; p < 0.001) and between active TB and non-MTB-infected children (AUC 0.82; sensitivity 88.0%; specificity 60.0%; p < 0.001). None of the biomarkers investigated were able to discriminate between LTBI and active TB. Conclusion: Our data suggest that combining the analyses of multiple biomarkers in plasma has the potential to enhance diagnosis of TB in children and, thus, warrants additional investigation. In particular, the diagnostic potential of IFN-γ/MCP-1 ratios should be further explored in larger pediatric cohorts. López-Suárez A, Santos-Sebastián M, Hernanz-Lobo A, Rincón-López E, Aguilera-Alonso D, Saavedra-Lozano J, Ruiz Serrano MJ, Hernández-Bartolomé Á, Medrano de Dios LM, Jiménez Fuentes JL, Navarro ML, Tebruegge M, Santiago-García B. Diagnostic potential of combining plasma biomarkers of tissue damage and inflammation in pediatric TB. J Microbiol Immunol Infect. 2024 Dec;57(6):937-946. 10.1016/j.jmii.2024.07.011 1995-9133 1684-1182 Journal of microbiology, immunology, and infection 39271436 https://hdl.handle.net/20.500.12105/26431 Biomarkers Diagnosis Pediatrics Tuberculosis Diagnostic potential of combining plasma biomarkers of tissue damage and inflammation in pediatric TB