2026-04-29T12:23:44Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/264302025-12-18T13:00:06Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Vidal-Alcántara, Erick Joan author Hernández Antón, Sonia author Rueda, Paloma author Yélamos, María Belén author Gómez, Julián author Resino, Salvador author Fresco-Taboada, Alba author Martinez, Isidoro author 2024-08-04 Background: Hepatitis C virus (HCV) infection remains a global health challenge, with millions of people affected annually. Current diagnostic methods, reliant on antibody screening and viral RNA detection, are complex, costly, and often inaccessible, particularly in resource-limited settings. Aim: Development of a lateral flow immunochromatography-based assay for detecting the highly conserved hepatitis C core antigen (HCVcAg). Methods: The assay relies on the interaction of four highly specific and cross-reactive monoclonal antibodies with recombinant HCVcAg from five different genotypes in a double antibody sandwich format. Latex and colloidal gold were evaluated as detector nanoparticles. Results: Extensive evaluation of 32 antibody combinations led to identifying the most sensitive antibody pairs. The chosen assay, named LN17, demonstrated a target sensitivity of 10 ng/strip, with potential clinical implications for detecting HCV. Furthermore, the study examined matrix effects in serum samples, providing valuable insights for future clinical application. Conclusions: The developed assay holds promise as a rapid, cost-effective, and user-friendly tool to enhance accessibility to hepatitis C screening, especially in high-risk populations and resource-limited environments. Vidal-Alcántara EJ, Antón SH, Rueda P, Yélamos MB, Gómez J, Resino S, Fresco-Taboada A, Martínez I. Development of a Lateral Flow Assay for the Detection of the Hepatitis C Virus Core Antigen. Pharmaceuticals (Basel). 2024 Aug 4;17(8):1022. 10.3390/ph17081022 1424-8247 Pharmaceuticals 39204127 https://hdl.handle.net/20.500.12105/26430 Core antigen Hepatitis C Monoclonal antibody Rapid diagnostic test Development of a Lateral Flow Assay for the Detection of the Hepatitis C Virus Core Antigen