2026-07-21T21:35:08Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/263522025-12-18T13:00:59Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Rejas-González, Raquel
author
Montero-Calle, Ana Maria
author
Valverde, Alejandro
author
Salvador, Natalia Pastora
author
Carballés, María José Crespo
author
Ausín-González, Emma
author
Sánchez-Naves, Juan
author
Campuzano, Susana
author
Barderas Manchado, Rodrigo
author
Guzman-Aranguez, Ana
funder
Complutense University of Madrid (España)
funder
Banco Santander
funder
Instituto de Salud Carlos III
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2025-02-21T13:54:59Z
2025-02-21T13:54:59Z
2024-06-26
Rejas-González R, Montero-Calle A, Valverde A, Salvador NP, Carballés MJC, Ausín-González E, Sánchez-Naves J, Campuzano S, Barderas R, Guzman-Aranguez A. Proteomics Analyses of Small Extracellular Vesicles of Aqueous Humor: Identification and Validation of GAS6 and SPP1 as Glaucoma Markers. Int J Mol Sci. 2024 Jun 26;25(13):6995.
10.3390/ijms25136995
1422-0067
International journal of molecular sciences
39000104
https://hdl.handle.net/20.500.12105/26352
Cataracts and glaucoma account for a high percentage of vision loss and blindness worldwide. Small extracellular vesicles (sEVs) are released into different body fluids, including the eye's aqueous humor. Information about their proteome content and characterization in ocular pathologies is not yet well established. In this study, aqueous humor sEVs from healthy individuals, cataracts, and glaucoma patients were studied, and their specific protein profiles were characterized. Moreover, the potential of identified proteins as diagnostic glaucoma biomarkers was evaluated. The protein content of sEVs from patients' aqueous humor with cataracts and glaucoma compared to healthy individuals was analyzed by quantitative proteomics. Validation was performed by western blot (WB) and ELISA. A total of 828 peptides and 192 proteins were identified and quantified. After data analysis with the R program, 8 significantly dysregulated proteins from aqueous humor sEVs in cataracts and 16 in glaucoma showed an expression ratio ≥ 1.5. By WB and ELISA using directly aqueous humor samples, the dysregulation of 9 proteins was mostly confirmed. Importantly, GAS6 and SPP1 showed high diagnostic ability of glaucoma, which in combination allowed for discriminating glaucoma patients from control individuals with an area under the curve of 76.1% and a sensitivity of 65.6% and a specificity of 87.7%.
eng
Aqueous humor
Cataracts
Glaucoma
Glaucoma diagnosis
Mass spectrometry
Proteomics
Small extracellular vesicles
Proteomics Analyses of Small Extracellular Vesicles of Aqueous Humor: Identification and Validation of GAS6 and SPP1 as Glaucoma Markers
research article
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