2026-07-21T20:33:51Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/263522025-12-18T13:00:59Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc Rejas-González, Raquel author Montero-Calle, Ana Maria author Valverde, Alejandro author Salvador, Natalia Pastora author Carballés, María José Crespo author Ausín-González, Emma author Sánchez-Naves, Juan author Campuzano, Susana author Barderas Manchado, Rodrigo author Guzman-Aranguez, Ana author 2024-06-26 Cataracts and glaucoma account for a high percentage of vision loss and blindness worldwide. Small extracellular vesicles (sEVs) are released into different body fluids, including the eye's aqueous humor. Information about their proteome content and characterization in ocular pathologies is not yet well established. In this study, aqueous humor sEVs from healthy individuals, cataracts, and glaucoma patients were studied, and their specific protein profiles were characterized. Moreover, the potential of identified proteins as diagnostic glaucoma biomarkers was evaluated. The protein content of sEVs from patients' aqueous humor with cataracts and glaucoma compared to healthy individuals was analyzed by quantitative proteomics. Validation was performed by western blot (WB) and ELISA. A total of 828 peptides and 192 proteins were identified and quantified. After data analysis with the R program, 8 significantly dysregulated proteins from aqueous humor sEVs in cataracts and 16 in glaucoma showed an expression ratio ≥ 1.5. By WB and ELISA using directly aqueous humor samples, the dysregulation of 9 proteins was mostly confirmed. Importantly, GAS6 and SPP1 showed high diagnostic ability of glaucoma, which in combination allowed for discriminating glaucoma patients from control individuals with an area under the curve of 76.1% and a sensitivity of 65.6% and a specificity of 87.7%. Rejas-González R, Montero-Calle A, Valverde A, Salvador NP, Carballés MJC, Ausín-González E, Sánchez-Naves J, Campuzano S, Barderas R, Guzman-Aranguez A. Proteomics Analyses of Small Extracellular Vesicles of Aqueous Humor: Identification and Validation of GAS6 and SPP1 as Glaucoma Markers. Int J Mol Sci. 2024 Jun 26;25(13):6995. 10.3390/ijms25136995 1422-0067 International journal of molecular sciences 39000104 https://hdl.handle.net/20.500.12105/26352 Aqueous humor Cataracts Glaucoma Glaucoma diagnosis Mass spectrometry Proteomics Small extracellular vesicles Proteomics Analyses of Small Extracellular Vesicles of Aqueous Humor: Identification and Validation of GAS6 and SPP1 as Glaucoma Markers