2026-05-23T02:49:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/263462025-12-18T13:00:37Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16973
Repisalud
author
Sancho-Albero, Maria
author
Sebastián, Víctor
author
Perez-Lopez, Ana M
author
Martin-Duque, Pilar
author
Unciti-Broceta, Asier
author
Santamaria, Jesus
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Asociación Española Contra el Cáncer
funder
Fundación Ramón Areces
funder
Fundación BBVA
funder
Engineering and Physical Sciences Research Council (Reino Unido)
2025-02-21T10:53:05Z
2025-02-21T10:53:05Z
2024-04-16
Sancho-Albero M, Sebastian V, Perez-Lopez AM, Martin-Duque P, Unciti-Broceta A, Santamaria J. Extracellular Vesicles-Mediated Bio-Orthogonal Catalysis in Growing Tumors. Cells. 2024 Apr 16;13(8):691.
10.3390/cells13080691
2073-4409
Cells
38667306
https://hdl.handle.net/20.500.12105/26346
Several studies have reported the successful use of bio-orthogonal catalyst nanoparticles (NPs) for cancer therapy. However, the delivery of the catalysts to the target tissues in vivo remains an unsolved challenge. The combination of catalytic NPs with extracellular vesicles (EVs) has been proposed as a promising approach to improve the delivery of therapeutic nanomaterials to the desired organs. In this study, we have developed a nanoscale bio-hybrid vector using a CO-mediated reduction at low temperature to generate ultrathin catalytic Pd nanosheets (PdNSs) as catalysts directly inside cancer-derived EVs. We have also compared their biodistribution with that of PEGylated PdNSs delivered by the EPR effect. Our results indicate that the accumulation of PdNSs in the tumour tissue was significantly higher when they were administered within the EVs compared to the PEGylated PdNSs. Conversely, the amount of Pd found in non-target organs (i.e., liver) was lowered. Once the Pd-based catalytic EVs were accumulated in the tumours, they enabled the activation of a paclitaxel prodrug demonstrating their ability to carry out bio-orthogonal uncaging chemistries in vivo for cancer therapy.
eng
Bio-orthogonal catalysis
Cancer
Extracellular vesicles
Palladium nanosheets
Extracellular Vesicles-Mediated Bio-Orthogonal Catalysis in Growing Tumors
research article
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ExtracellularVesicles-MediatedBio-Orthogonal_2024.pdf
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Supplementary_ExtracellularVesicles-MediatedBio-Orthogonal_2024.pdf