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oai:repisalud.isciii.es:20.500.12105/263462025-12-18T13:00:37Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16973

00925njm 22002777a 4500 dc Sancho-Albero, Maria author Sebastián, Víctor author Perez-Lopez, Ana M author Martin-Duque, Pilar author Unciti-Broceta, Asier author Santamaria, Jesus author 2024-04-16 Several studies have reported the successful use of bio-orthogonal catalyst nanoparticles (NPs) for cancer therapy. However, the delivery of the catalysts to the target tissues in vivo remains an unsolved challenge. The combination of catalytic NPs with extracellular vesicles (EVs) has been proposed as a promising approach to improve the delivery of therapeutic nanomaterials to the desired organs. In this study, we have developed a nanoscale bio-hybrid vector using a CO-mediated reduction at low temperature to generate ultrathin catalytic Pd nanosheets (PdNSs) as catalysts directly inside cancer-derived EVs. We have also compared their biodistribution with that of PEGylated PdNSs delivered by the EPR effect. Our results indicate that the accumulation of PdNSs in the tumour tissue was significantly higher when they were administered within the EVs compared to the PEGylated PdNSs. Conversely, the amount of Pd found in non-target organs (i.e., liver) was lowered. Once the Pd-based catalytic EVs were accumulated in the tumours, they enabled the activation of a paclitaxel prodrug demonstrating their ability to carry out bio-orthogonal uncaging chemistries in vivo for cancer therapy. Sancho-Albero M, Sebastian V, Perez-Lopez AM, Martin-Duque P, Unciti-Broceta A, Santamaria J. Extracellular Vesicles-Mediated Bio-Orthogonal Catalysis in Growing Tumors. Cells. 2024 Apr 16;13(8):691. 10.3390/cells13080691 2073-4409 Cells 38667306 https://hdl.handle.net/20.500.12105/26346 Bio-orthogonal catalysis Cancer Extracellular vesicles Palladium nanosheets Extracellular Vesicles-Mediated Bio-Orthogonal Catalysis in Growing Tumors