2026-07-22T03:37:00Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/263412025-12-18T12:59:19Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Tejerina-Miranda, Sandra
author
Pérez-Ginés, Víctor
author
Torrente-Rodríguez, Rebeca M
author
Pedrero, María
author
Montero-Calle, Ana Maria
author
Pingarrón, José M
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
funder
Agencia Estatal de Investigación (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Comisión Europea. H2020
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Complutense University of Madrid (España)
2025-02-17T14:13:56Z
2025-02-17T14:13:56Z
2024
Magneto-controlled electrochemical immunosensing platform to assess the senescence-associated GDF-15 marker in colorectal cancer. Sens. Diagn.2024,3:238-247.
10.1039/d3sd00311f
2635-0998
Sensors & Diagnostics
https://hdl.handle.net/20.500.12105/26341
In this study, we report a novel electrochemical immunoplatform using magnetic micro-supports and screen-printed carbon electrodes (SPCEs), overcoming limitations of the methods reported to date, for the rapid and sensitive determination of GDF-15, a molecular marker associated with cellular senescence in aging and cancer development and prognosis. The immunoplatform incorporated a sandwich-type configuration with specific capture and biotinylated-detection antibodies and used a streptavidin–peroxidase (strep–HRP) enzymatic conjugate as label. After magnetic capturing the micro-supports with the sandwich HRP-labeled immunocomplexes onto the surface of a SPCE, the change in cathodic current was quantified in the presence of H2O2 and hydroquinone (HQ), showing a direct correlation with the GDF-15 concentration. The proposed bioplatform exhibited attractive performance characteristics, including a good reproducibility (RSD 4.3%), and a wide linear concentration dynamic range from 140 to 10 000 pg mL−1 with a low limit of detection (LOD) of 42 pg mL−1 for GDF-15 standards in buffered solutions. The selectivity of the developed method and the storage stability of the capturing immunoconjugates were noteworthy. Indeed, the immunoconjugates showed a reliable performance for over 28 days when stored in a refrigerator. The immunoplatform was applied to a cohort of 19 plasma samples representing the different stages of colorectal cancer (CRC). The method allowed efficient discrimination between healthy individuals and CRC patients, particularly those in advanced stages, within a rapid 75-minute timeframe. The immunoplatform also presents substantial advantages in terms of cost-effectiveness, assay time reduction, and simplicity compared to other available techniques.
eng
Colorectal cancer
Screen-printed carbon electrodes
Electrochemical immunoplatforms
GDF-15
Magneto-controlled electrochemical immunosensing platform to assess the senescence-associated GDF-15 marker in colorectal cancer
research article
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URL
https://repisalud.isciii.es/bitstreams/0b70b6aa-cd3b-46c7-93c0-92868c5d2fb2/download
File
MD5
a806f90d586cad52ba67c26e66fa01a6
1803658
application/pdf
Magneto-controlledElectrochemicalImmunosensing_2024.pdf