2026-05-20T23:31:38Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/262952025-12-18T12:59:38Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Sempere, Julio author Yuste, Jose Enrique author Domenech Lucas, Mirian author 2024-11 In respiratory pathogens such as , biofilm formation is associated with the colonization of the nasopharynx and chronic respiratory infection. Previous data have shown that pneumococcal conjugate vaccines (PCVs) had an impact on colonization and a potential replacement by other respiratory pathogens such as . The objective of this work was to evaluate the evasion of the immune system by monospecific biofilms and by mixed biofilms. We performed opsonophagocytosis assays (OPA) using human HL-60 against previously disaggregated monospecific biofilms of MSSA, MRSA and mixed biofilms. We used pre-immune and post-immune serum from immunocompetent adult patients vaccinated with PCV13. Immune sera had a clear effect in reducing pneumococcal biofilms of serotypes 3, 14, 18C, 19F and 19A, whereas had no effect in non-PCV13 serotypes such as 8, 11A and 24F. Our study confirmed that serum from vaccinated patients with PCV13 did not have any effect in reducing population in monospecific biofilms, regardless the methicillin resistance phenotype. Moreover, immunized sera from vaccinated patients with PCV13 did not have any effect in population in the mixed biofilm, whereas significantly reduced the population of pneumococcal serotype 19A strain in the mixed biofilm which is of great interest because this serotype is included in PCV13, and it is associated with vaccine failures. Sempere J, Yuste J, Domenech M. PCV13 vaccine prevents pneumococcal biofilms without affecting Staphylococcus aureus population within the polymicrobial biofilm. Front Immunol. 2024 Nov 1;15:1495932. 10.3389/fimmu.2024.1495932 1664-3224 Frontiers in immunology 39555077 https://hdl.handle.net/20.500.12105/26295 MRSA OPA PCV13 S. aureus S. pneumoniae Biofilms PCV13 vaccine prevents pneumococcal biofilms without affecting population within the polymicrobial biofilm