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oai:repisalud.isciii.es:20.500.12105/262602025-12-18T12:59:57Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16982
Repisalud author Köster, Pamela Carolina author Figueiredo, Ana M author Maloney, Jenny G author Dashti, Alejandro author Bailo-Barroso, Begoña author Torres, Rita T author Fonseca, Carlos author Mysterud, Atle author Habela, Miguel Ángel author Rivero-Juarez, Antonio author Vicente, Joaquín author Serrano, Emmanuel author Arnal, Maria C author Fernández de Luco, Daniel author Armenteros, José A author Balseiro, Ana author Cardona, Guillermo A author Carvalho, João author Hipólito, Dário author Fernandes, Joana author Palmeira, Josman D author Calero-Bernal, Rafael author Gonzalez-Barrio, David author Santín, Mónica author Carmena, David funder Instituto de Salud Carlos III funder Ministerio de Economía y Competitividad (España) funder United States Department of Agriculture funder Fundação para a Ciência e Tecnologia (Portugal) funder Unión Europea. Fondo Social Europeo (ESF/FSE) funder Ministerio de Ciencia, Innovación y Universidades (España) funder Unión Europea. European Cooperation in Science and Technology (COST) 2025-02-05T09:25:06Z 2025-02-05T09:25:06Z 2024-10-07 Köster PC, Figueiredo AM, Maloney JG, Dashti A, Bailo B, Torres RT, Fonseca C, Mysterud A, Habela MÁ, Rivero-Juarez A, Vicente J, Serrano E, Arnal MC, de Luco DF, Armenteros JA, Balseiro A, Cardona GA, Carvalho J, Hipólito D, Fernandes J, Palmeira JD, Calero-Bernal R, González-Barrio D, Santin M, Carmena D. Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula. Vet Res. 2024 Oct 7;55(1):133. doi: 10.1186/s13567-024-01385-9. Erratum in: Vet Res. 2024 Nov 14;55(1):152. 10.1186/s13567-024-01385-9 1297-9716 0928-4249 Veterinary research 39375799 https://hdl.handle.net/20.500.12105/26260 The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and is observed in humans and animals worldwide. The role of wildlife in the epidemiology of Blastocystis is insufficiently understood. Thus, we investigated the occurrence and subtype diversity of Blastocystis in free-ranging wild boars from the Iberian Peninsula using conventional PCR and next-generation amplicon sequencing of a fragment of the ssu RNA gene. A total of 459 wild boar faecal samples were collected across Spain (n = 360) and Portugal (n = 99) between 2014 and 2021. Blastocystis was present in 15.3% (70/459; 95% CI 12.1-18.9) of the wild boars analysed, and its occurrence was significantly higher in Portugal (34.3%, 34/99; 95% CI 25.1-44.6) than in Spain (10.0%, 36/360; 95% CI 7.1-13.6). Seven Blastocystis subtypes (ST5, ST10b, ST13-ST15, ST24b, and ST43) were detected among the surveyed wild boar populations, with greater variability detected in Portuguese samples. ST5 was identified in all the Blastocystis-positive animals, whereas 14.3% of them harboured ST mixed colonisations. Our results demonstrate that Blastocystis ST5 is particularly adapted to infect wild boars. The additional identification of zoonotic STs reinforces the role of wild boars as spreaders of zoonotic infections with public health significance. eng Epidemiology NGS Portugal Spain Subtype diversity wildlife Zoonoses Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula. research article 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 URL https://repisalud.isciii.es/bitstreams/bc1ebbf3-72a2-40f1-8e42-aa2ccb7849d4/download File MD5 85252b7ab1350bd4e6bace8163975f60 1330800 application/pdf BlastocystisOccurrenceSubtypeDiversity_2024.pdf URL https://repisalud.isciii.es/bitstreams/6729b757-6a78-4562-bcfb-821bce6d42cc/download File MD5 21dce3c775120636d2a3e6edbbb96d91 210707 application/pdf Supplementary1_BlastocystisOccurrenceSubtypeDiversity_2024.pdf URL https://repisalud.isciii.es/bitstreams/24ec25b9-b2e4-4ec4-9c87-67d7b6a4f620/download File MD5 b6f7b7de8d160951b3cb8c30399e7e8e 51084 application/vnd.openxmlformats-officedocument.spreadsheetml.sheet Supplementary2_BlastocystisOccurrenceSubtypeDiversity_2024.xlsx URL https://repisalud.isciii.es/bitstreams/e9c21be1-1fef-47cf-9177-08c6be4900fc/download File MD5 8010bcc81d446d184b2071f5969664be 739159 application/pdf Erratum_BlastocystisOccurrenceSubtypeDiversity_2024.pdf