2026-04-29T04:09:50Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/262502025-12-18T13:00:42Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Lebbad, Marianne author Grüttner, Jana author Beser, Jessica author Lizana, Victor author Dea-Ayuela, María Auxiliadora author Oropeza-Moe, Marianne author Carmena, David author Stensvold, Christen Rune author 2024-08 Cryptosporidiosis is an infectious enteric disease caused by species (some of them zoonotic) of the genus Cryptosporidium that in many countries are under surveillance. Typing assays critical to the surveillance of cryptosporidiosis typically involve characterization of Cryptosporidium glycoprotein 60 genes (gp60). Here, we characterized the gp60 of Cryptosporidium suis from two samples-a human and a porcine faecal sample-based on which a preliminary typing scheme was developed. A conspicuous feature of the C. suis gp60 was a novel type of tandem repeats located in the 5' end of the gene and that took up 777/1635 bp (48%) of the gene. The C. suis gp60 lacked the classical poly-serine repeats (TCA/TCG/TCT), which is usually subject to major genetic variation, and the length of the tandem repeat made a typing assay incorporating this region based on Sanger sequencing practically unfeasible. We therefore designed a typing assay based on the post-repeat region only and applied it to C. suis-positive samples from suid hosts from Norway, Denmark, and Spain. We were able to distinguish three different subtypes; XXVa-1, XXVa-2, and XXVa-3. Subtype XXVa-1 had a wider geographic distribution than the other subtypes and was also observed in the human sample. We think that the present data will inform future strategies to develop a C. suis typing assay that could be even more informative by including a greater part of the gene, including the tandem repeat region, e.g., by the use of long-read next-generation sequencing. Lebbad M, Grüttner J, Beser J, Lizana V, Dea-Ayuela MA, Oropeza-Moe M, Carmena D, Stensvold CR. Complete sequencing of the Cryptosporidium suis gp60 gene reveals a novel type of tandem repeats-Implications for surveillance. Infect Genet Evol. 2024 Aug;122:105614. 1567-1348 https://hdl.handle.net/20.500.12105/26250 38844191 10.1016/j.meegid.2024.105614 1567-7257 Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases Epidemiology Genetic diversity Host specificity Molecular epidemiology Protozoa Zoonosis Complete sequencing of the Cryptosporidium suis gp60 gene reveals a novel type of tandem repeats-Implications for surveillance