2026-04-26T23:12:46Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/262222025-12-18T12:52:18Zcom_20.500.12105_2327com_20.500.12105_2290col_20.500.12105_16991

00925njm 22002777a 4500 dc Martinez-Pastor, Barbara author Silveira, Giorgia G author Clarke, Thomas L author Chung, Dudley author Gu, Yuchao author Cosentino, Claudia author Davidow, Lance S author Mata, Gadea author Hassanieh, Sylvana author Salsman, Jayme author Ciccia, Alberto author Bae, Narkhyun author Bedford, Mark T author Megias, Diego author Rubin, Lee L author Efeyan, Alejo author Dellaire, Graham author Mostoslavsky, Raul author 2021-12-28 Repair of genetic damage is coordinated in the context of chromatin, so cells dynamically modulate accessibility at DNA breaks for the recruitment of DNA damage response (DDR) factors. The identification of chromatin factors with roles in DDR has mostly relied on loss-of-function screens while lacking robust high-throughput systems to study DNA repair. In this study, we have developed two high-throughput systems that allow the study of DNA repair kinetics and the recruitment of factors to double-strand breaks in a 384-well plate format. Using a customized gain-of-function open-reading frame library ("ChromORFeome" library), we identify chromatin factors with putative roles in the DDR. Among these, we find the PHF20 factor is excluded from DNA breaks, affecting DNA repair by competing with 53BP1 recruitment. Adaptable for genetic perturbations, small-molecule screens, and large-scale analysis of DNA repair, these resources can aid our understanding and manipulation of DNA repair. Cell Rep . 2021 Dec 28;37(13):110176 https://hdl.handle.net/20.500.12105/26222 34965416 Cell Rep DNA damage foci DNA repair GOF PHF20 chromatin accessibility high throughput laser microirradiation Assessing kinetics and recruitment of DNA repair factors using high content screens.