2026-08-29T14:55:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/262162025-12-18T12:56:57Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Zhong, Lei
author
Martinez-Pastor, Barbara
author
Silberman, Dafne M
author
Sebastian, Carlos
author
Mostoslavsky, Raul
2025-01-31T11:48:56Z
2025-01-31T11:48:56Z
2013
Methods Mol Biol . 2013:1077:149-163
Methods in Mol Biol
https://pmc.ncbi.nlm.nih.gov/articles/PMC4605221/pdf/nihms-725238.pdf
24014405
https://hdl.handle.net/20.500.12105/26216
Most of the sirtuins' nuclear substrates identified so far are histones or other chromatin-associated proteins and, thus, it is of special relevance the development of good biochemical techniques to analyze the biology of these proteins in the context of chromatin. Here, we describe several of the chromatin-based techniques to identify sirtuins' substrates, including a chromatin immunoprecipitation (ChIP) protocol, an acid-extraction protocol, and a nucleosomal immunoprecipitation protocol to analyze putative sirtuin chromatin interactors.
eng
Sirtuins
Chromatin
Chromatin immunoprecipitation
ChIP
Acid-extraction
Nucleosomal immunoprecipitation
Assaying chromatin sirtuins.
research article
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URL
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7499308
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assayingchromatin-2013.pdf