2026-08-29T14:55:54Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/262162025-12-18T12:56:57Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Zhong, Lei author Martinez-Pastor, Barbara author Silberman, Dafne M author Sebastian, Carlos author Mostoslavsky, Raul author 2013 Most of the sirtuins' nuclear substrates identified so far are histones or other chromatin-associated proteins and, thus, it is of special relevance the development of good biochemical techniques to analyze the biology of these proteins in the context of chromatin. Here, we describe several of the chromatin-based techniques to identify sirtuins' substrates, including a chromatin immunoprecipitation (ChIP) protocol, an acid-extraction protocol, and a nucleosomal immunoprecipitation protocol to analyze putative sirtuin chromatin interactors. Methods Mol Biol . 2013:1077:149-163 Methods in Mol Biol https://pmc.ncbi.nlm.nih.gov/articles/PMC4605221/pdf/nihms-725238.pdf 24014405 https://hdl.handle.net/20.500.12105/26216 Sirtuins Chromatin Chromatin immunoprecipitation ChIP Acid-extraction Nucleosomal immunoprecipitation Assaying chromatin sirtuins.