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oai:repisalud.isciii.es:20.500.12105/262132025-12-18T12:52:22Zcom_20.500.12105_2273com_20.500.12105_2202col_20.500.12105_19571

00925njm 22002777a 4500 dc Miranda-Lorenzo, Irene author Dorado, Jorge author Lonardo, Enza author Alcala, Sonia author Serrano, Alicia G author Clausell-Tormos, Jenifer author Cioffi, Michele author Megias, Diego author Zagorac, Sladjana author Balic, Anamaria author Hidalgo, Manuel author Erkan, Mert author Kleeff, Joerg author Scarpa, Aldo author Sainz, Bruno author Heeschen, Christopher author 2014-11 Cancer stem cells (CSCs) are thought to drive tumor growth, metastasis and chemoresistance. Although surface markers such as CD133 and CD44 have been successfully used to isolate CSCs, their expression is not exclusively linked to the CSC phenotype and is prone to environmental alteration. We identified cells with an autofluorescent subcellular compartment that exclusively showed CSC features across different human tumor types. Primary tumor-derived autofluorescent cells did not overlap with side-population (SP) cells, were enriched in sphere culture and during chemotherapy, strongly expressed pluripotency-associated genes, were highly metastatic and showed long-term in vivo tumorigenicity, even at the single-cell level. Autofluorescence was due to riboflavin accumulation in membrane-bounded cytoplasmic structures bearing ATP-dependent ABCG2 transporters. In summary, we identified and characterized an intrinsic autofluorescent phenotype in CSCs of diverse epithelial cancers and used this marker to isolate and characterize these cells. Nat Methods . 2014 Nov;11(11):1161-9 25262208 https://hdl.handle.net/20.500.12105/26213 RESISTANCE PROTEIN PANCREATIC-CANCER SIDE POPULATION ADENOCARCINOMA IDENTIFICATION SECRETION GROWTH MILK Intracellular autofluorescence: a biomarker for epithelial cancer stem cells.