2026-05-22T00:30:05Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/261882025-12-18T12:59:37Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Muñoz-Chimeno, Milagros author Díaz-Sánchez, Nazaret author Morago, Lucia author Rodriguez-Paredes, Vanessa author Barturen, Silvia author Rodriguez-Recio, Alvaro author Garcia-Lugo, Maira Alejandra author Zamora, Maria Isabel author Mateo, María author Sanchez-Martinez, Monica author Avellón, Ana author 2024-09-11 HEV antibody detection constitutes the main screening test for HEV infection. The aim of this study is to compare the sensitivity and specificity of four techniques: LIAISON® MUREX DiaSorin anti-HEV IgG and anti-HEV IgM assays, Hepatitis E VIRCLIA® IgM and IgG monotests, WANTAI HEV-IgM and IgG ELISA and VIDAS® anti-HEV IgM and IgG tests in five panels of samples configurated according to the immunoblot (RecomLine, Mikrogen, Neuss, Germany). Anti-HEV IgM sensitivity in the acute phase was 100% in all techniques, while sensitivity, including the immediate convalescence phase, was 96.74% for LIAISON®, 83.14% for VIRCLIA®, 84.78% for WANTAI and 88.04% for VIDAS®. Anti-HEV IgM specificity was 100% for both LIAISON® and VIRCLIA®. Anti-HEV IgM WANTAI agreed with VIRCLIA® with a good Kappa coefficient (κ = 0.71). Anti-HEV IgG post-infection sensitivity was 100% for LIAISON®, VIDAS® and VIRCLIA® and 99% for WANTAI. Anti-HEV IgG specificity reached 97.17% for LIAISON and 88.68% for VIRCLIA®. Our results demonstrated a better capacity of LIAISON® MUREX anti-HEV IgM than that of competitors for detecting acute infections as well as accurate anti-HEV IgG results and in how to resolve them. Muñoz-Chimeno M, Díaz-Sánchez N, Morago L, Rodríguez-Paredes V, Barturen S, Rodríguez-Recio Á, García-Lugo MA, Zamora MI, Mateo M, Sánchez-Martínez M, Avellón A. Performance Comparison of Four Hepatitis E Antibodies Detection Methods. Microorganisms. 2024 Sep 11;12(9):1875. 10.3390/microorganisms12091875 2076-2607 Microorganisms 39338549 https://hdl.handle.net/20.500.12105/26188 HEV IgG IgM anti-HEV Antibody Chemiluminescence Hepatitis E virus Performance Comparison of Four Hepatitis E Antibodies Detection Methods