2026-08-29T14:55:24Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/261532025-12-18T13:00:49Zcom_20.500.12105_19586com_20.500.12105_2202col_20.500.12105_19587
Repisalud
author
Torres, Raul
author
Ramirez, Juan C
funder
Ministerio de Sanidad y Consumo (España)
funder
Fundación Mutua Madrileña
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación ProCNIC
2025-01-27T14:14:20Z
2025-01-27T14:14:20Z
2009-10-27
PLoS One. 2009 Oct 27;4(10):e7570.
PLoS One
19859557
https://hdl.handle.net/20.500.12105/26153
Chemokines are extracellular mediators of complex regulatory circuits involved principally in cell-to-cell communication. Most studies to date of the essential chemokine Cxcl12 (Sdf-1) have focused on the ubiquitously expressed secreted isoforms alpha and beta. Here we show that, unlike these isoforms and all other known chemokines, the alternatively transcribed gamma isoform is an intracellular protein that localizes to the nucleolus in differentiated mouse Cardiac tissue. Our results demonstrate that nucleolar transportation is encoded by a nucleolar-localization signal in the unique carboxy-terminal region of Sdf-1gamma, and is competent both in vivo and in vitro. The molecular mechanism underlying these unusual chemokine properties involves cardiac-specific transcription of an mRNA containing a unique short-leader sequence lacking the signal peptide and translation from a non-canonical CUG codon. Our results provide an example of genome economy even for essential and highly conserved genes such as Cxcl12, and suggest that chemokines can exert tissue specific functions unrelated to cell-to-cell communication.
eng
A chemokine targets the nucleus: Cxcl12-gamma isoform localizes to the nucleolus in adult mouse heart.
research article
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URL
https://repisalud.isciii.es/bitstreams/f1dfcaea-5543-4a25-8311-049524bca29c/download
File
MD5
b90875ff1ea9dc1a7923dd54b8f13ee1
2112149
application/pdf
A Chemokine Targets_PLoS One_2009.pdf