2026-06-14T03:29:05Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/261512025-12-18T12:56:02Zcom_20.500.12105_2327com_20.500.12105_2290col_20.500.12105_16991
Repisalud
author
Torres-Ruiz, Raul
author
Martin, M C
author
Garcia, A
author
Cigudosa, Juan C
author
Ramirez, J C
author
Rodriguez Perales, Sandra
funder
Pro-CNIC Foundation
funder
INTRASALUD
funder
Ministerio de Economia y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Red Tematica de Investigacion Cooperativa en Cancer (RTICC)
2025-01-27T12:39:59Z
2025-01-27T12:39:59Z
2014-06-03
Nat Commun . 2014 Jun 3:5:3964.
Nature Communications
24888982
https://hdl.handle.net/20.500.12105/26151
Cancer-related human chromosomal translocations are generated through the illegitimate joining of two non-homologous chromosomes affected by double-strand breaks (DSB). Effective methodologies to reproduce precise reciprocal tumour-associated chromosomal translocations are required to gain insight into the initiation of leukaemia and sarcomas. Here we present a strategy for generating cancer-related human chromosomal translocations in vitro based on the ability of the RNA-guided CRISPR-Cas9 system to induce DSBs at defined positions. Using this approach we generate human cell lines and primary cells bearing chromosomal translocations resembling those described in acute myeloid leukaemia and Ewing's sarcoma at high frequencies. FISH and molecular analysis at the mRNA and protein levels of the fusion genes involved in these engineered cells reveal the reliability and accuracy of the CRISPR-Cas9 approach, providing a powerful tool for cancer studies.
eng
REARRANGEMENTS
RECOMBINATION
CRISPR/CAS
GENES
CELLS
Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR-Cas9 system.
research article
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URL
https://repisalud.isciii.es/bitstreams/009587bf-62b8-411c-a70e-f74318dc9b19/download
File
MD5
1d6f51932f827f8d2d257b9df62835d6
1098510
application/pdf
enginneringhumantunour-2014.pdf