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oai:repisalud.isciii.es:20.500.12105/261272025-12-18T12:57:05Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Sánchez-Molina, Sara author Oliva-Martinez, Jose Luis author Garcia-Vargas, Susana author Valls, Ester author Rojas-Cabañeros, Jose Maria author Martínez-Balbás, Marian A author 2006-09-01 The CBP [CREB (cAMP-response-element-binding protein)-binding protein]/p300 acetyltransferases function as transcriptional co-activators and play critical roles in cell differentiation and proliferation. Accumulating evidence shows that alterations of the CBP/p300 protein levels are linked to human tumours. In the present study, we show that the levels of the CBP/p300 co-activators are decreased dramatically by continuous PDGF (platelet-derived growth factor) and Ras signalling pathway activation in NIH 3T3 fibroblasts. This effect occurs by reducing the expression levels of the CBP/p300 genes. In addition, CBP and p300 are degraded by the 26 S proteasome pathway leading to an overall decrease in the levels of the CBP/p300 proteins. Furthermore, we provide evidence that Mdm2 (murine double minute 2), in the presence of active H-Ras or N-Ras, induces CBP/p300 degradation in NIH 3T3 cells. These findings support a novel mechanism for modulating other signalling transduction pathways that require these common co-activators. Sánchez-Molina S, Oliva JL, García-Vargas S, Valls E, Rojas JM, Martínez-Balbás MA. The histone acetyltransferases CBP/p300 are degraded in NIH 3T3 cells by activation of Ras signalling pathway. Biochem J. 2006 Sep 1;398(2):215-24. 10.1042/BJ20060052 1470-8728 0264-6021 The Biochemical journal https://pmc.ncbi.nlm.nih.gov/articles/PMC1550303/ 16704373 https://hdl.handle.net/20.500.12105/26127 Acetylation cAMP-response-element-binding-protein-binding protein/p300 (CBP/p300) Histone acetyltransferase activity (HAT activity) Murine double minute 2 (Mdm2) NIH 3T3 cell Ras pathway The histone acetyltransferases CBP/p300 are degraded in NIH 3T3 cells by activation of Ras signalling pathway.