2026-05-22T00:40:21Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/261182025-12-18T13:00:50Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Álvarez Flores, María Beatriz
author
Sopeña Corvinos, María
author
Guillén Santos, Raquel
author
Cava Valenciano, Fernando
funder
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
2025-01-23T16:02:15Z
2025-01-23T16:02:15Z
2024-12-22
Diseases. 2024 Dec 22;12(12):338.
Diseases
39727668
https://hdl.handle.net/20.500.12105/26118
Monitoring of measurable residual disease (MRD) requires highly sensitive flow cytometry protocols to provide an accurate prediction of shorter progression-free survival. High assay sensitivity generally requires rapid processing to avoid cell loss from small bone marrow sample volumes, but this requirement conflicts with the need in most clinical cytometry laboratories for long processing and acquisition times, especially when multiple MRD studies coincide on the same day. The proposed protocol was applied to 226 human bone marrow and 45 peripheral blood samples submitted for the study of MRD or the detection of rare cells. Samples were processed within 24 h of extraction and acquired with an eight-color flow cytometer. The FACSLyse-Bulk protocol allows for the labelling of millions of cells in under 90 min in small sample volumes without affecting the FSC/SSC pattern or antigen expression, and it also allows antigens to be fixed to the membrane, thus avoiding the capping phenomenon. The proposed protocol would allow clinical flow cytometry laboratories to perform MRD studies in house and easily achieve a limit of detection and limit of quantification <0.001%, thus avoiding the need to outsource analysis to specialized cytometry laboratories.
eng
bone marrow
flow cytometry
hematology
high-sensitivity flow cytometry
leukemia
limit of detection
lymphoma
mastocytosis
measurable residual disease
multiple myeloma
staining and labeling
High-Sensitivity Flow Cytometry for the Reliable Detection of Measurable Residual Disease in Hematological Malignancies in Clinical Laboratories.
research article
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URL
https://repisalud.isciii.es/bitstreams/ede13b59-e352-4059-b7e0-9af38efe8122/download
File
MD5
95e0c76829394b16416d4eaa3c293ec6
3832964
application/pdf
High Sensitivity Flow Cytometry_Diseases_2024.pdf