2026-04-26T23:12:46Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/261152025-12-18T12:54:29Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Oliva-Martinez, Jose Luis author Pérez-Sala, Dolores author Castrillo, Antonio author Martinez, Natalia author Cañada, F Javier author Boscá, Lisardo author Rojas-Cabañeros, Jose Maria author 2003-04-15 The cyclopentenone 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) induces cell proliferation and mitogen-activated protein kinase activation. Here, we describe that these effects are mediated by 15d-PGJ(2)-elicited H-Ras activation. We demonstrate that this pathway is specific for H-Ras through the formation of a covalent adduct of 15d-PGJ(2) with Cys-184 of H-Ras, but not with N-Ras or K-Ras. Mutation of C184 inhibited H-Ras modification and activation by 15d-PGJ(2), whereas serum-elicited stimulation was not affected. These results describe a mechanism for the activation of the Ras signaling pathway, which results from the chemical modification of H-Ras by formation of a covalent adduct with cyclopentenone prostaglandins. Oliva JL, Pérez-Sala D, Castrillo A, Martínez N, Cañada FJ, Boscá L, Rojas JM. The cyclopentenone 15-deoxy-delta 12,14-prostaglandin J2 binds to and activates H-Ras. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4772-7. 0027-8424 https://pmc.ncbi.nlm.nih.gov/articles/PMC153631/ https://hdl.handle.net/20.500.12105/26115 12684535 10.1073/pnas.0735842100 1091-6490 Proceedings of the National Academy of Sciences of the United States of America Mitogen-activated protein kinase Cell proliferation Posttranslational modification The cyclopentenone 15-deoxy-delta 12,14-prostaglandin J2 binds to and activates H-Ras