2026-04-29T07:25:10Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/260602025-12-18T13:00:00Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Noro, Fabrizia author Santonastaso, Federica author Marotta, Annalisa author Bonaccio, Marialaura author Orlandi, Sabatino author Tirozzi, Alfonsina author Costanzo, Simona author De Curtis, Amalia author Gianfagna, Francesco author Di Castelnuovo, Augusto author Brighenti, Furio author Cerletti, Chiara author Donati, Maria Benedetta author de Gaetano, Giovanni author Iacoviello, Licia author Gialluisi, Alessandro author Izzi, Benedetta author 2022-12-28 High dietary glycaemic index (GI) and load (GL) have been associated with increased risk of various cardiometabolic conditions. Among the molecular potential mechanisms underlying this relationship, DNA methylation has been studied, but a direct link between high GI and/or GL of diet and global DNA methylation levels has not been proved yet. We analyzed the associations between GI and GL and global DNA methylation patterns within an Italian population. Genomic DNA methylation (5mC) and hydroxymethylation (5hmC) levels were measured in 1080 buffy coat samples from participants of the Moli-sani study (mean(SD) = 54.9(11.5) years; 52% women) via ELISA. A 188-item Food Frequency Questionnaire was used to assess food intake and dietary GI and GL for each participant were calculated. Multiple linear regressions were used to investigate the associations between dietary GI and GL and global 5mC and 5hmC levels, as well as the proportion of effect explained by metabolic and inflammatory markers. We found negative associations of GI with both 5mC (β (SE) = - 0.073 (0.027), p = 0.007) and 5hmC (- 0.084 (0.030), p = 0.006), and of GL with 5mC (- 0.14 (0.060), p = 0.014). Circulating biomarkers did not explain the above-mentioned associations. Gender interaction analyses revealed a significant association of the gender-x-GL interaction with 5mC levels, with men showing an inverse association three times as negative as in women (interaction β (SE) = - 0.16 (0.06), p = 0.005). Our findings suggest that global DNA methylation and hydroxymethylation patterns represent a biomarker of carbohydrate intake. Based on the differential association of GL with 5mC between men and women, further gender-based separate approaches are warranted. Clin Epigenetics. 2022 Dec 28;14(1):189. https://hdl.handle.net/20.500.12105/26060 36578055 Clinical Epigenetics Glycaemic index Glycaemic load Hydroxymethylation Metabolic disorders Methylation Association of nutritional glycaemic indices with global DNA methylation patterns: results from the Moli-sani cohort.