2026-08-29T09:10:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/260552025-12-18T13:01:52Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Marotta, Annalisa
author
Noro, Fabrizia
author
Parisi, Roberta
author
Gialluisi, Alessandro
author
Tirozzi, Alfonsina
author
De Curtis, Amalia
author
Costanzo, Simona
author
Di Castelnuovo, Augusto
author
Cerletti, Chiara
author
Donati, Maria Benedetta
author
de Gaetano, Giovanni
author
Iacoviello, Licia
author
Izzi, Benedetta
author
Gianfagna, Francesco
funder
Fundación Pfizer
funder
Fondazione Umberto Veronesi
2025-01-17T13:37:24Z
2025-01-17T13:37:24Z
2021-12
Epigenetics. 2021 Dec;16(12):1347-1360.
Epigenetics
33393847
https://hdl.handle.net/20.500.12105/26055
Neuromedin U (NMU) is a neuropeptide involved in gut-brain axis, energy balance and immune response. We aimed at analysing the association between epigenetic variability and metabolic indices and the potential mediating role of low-grade inflammation in a general population of Italian adults. Blood DNA methylation levels at two CpG islands ( and ) were analysed using pyrosequencing in a randomly selected sub-cohort of 1,160 subjects from the Moli-sani study (≥35years; 49.20% men). Multivariable regressions adjusted for age, sex, smoking, alcohol and vegetable consumption were performed to estimate the associations between methylation and metabolic phenotypes (BMI, waist-to-hip ratio, blood pressure, glucose, HOMA-IR, lipids, lipoprotein(a) and apolipoproteins). Mediation analysis was performed to identify the influence of low-grade inflammation in the association using a composite index based on C reactive protein, granulocyte-to-lymphocyte ratio (GLR), platelet and white blood cell counts (INFLA-score).Using principal component analysis four methylation factors were identified: and . was FDR significantly associated with total cholesterol (for 1 SD increase: β = 4.5 ± 1.4 mg/dL of, R = 10.8%, p = 0.001), ApoB (0.03 ± 0.01 g/L, 12.2%, p = 0.0004), with INFLA-score (1.05 ± 0.22, = 2.7E-6) and GLR (-0.27 ± 0.03, 30.4%, = 1.3E-20). GLR and lymphocyte numbers mediate the association of with cholesterol (24.0% of total effect, Sobel p = 0.013) and ApoB (42.6%, p = 9E-7), respectively.These findings suggest that promoter methylation patterns could mark a pathway linking lipids with haematopoiesis and systemic inflammation.
eng
DNA methylation
apolipoprotein B
cardiovascular risk
granulocyte-to-lymphocyte ratio
low-grade inflammation
metabolic indices
neuromedin U
white blood cell count
DNA methylation in blood is associated with metabolic and inflammatory indices: results from the Moli-sani study.
research article
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URL
https://repisalud.isciii.es/bitstreams/870fb5a0-36df-4604-94a2-788a3485f11e/download
File
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df1a2691996bbdf9d168b67ed2a772d2
2173683
application/pdf
NMU DNA methylation in blood_Epigenetics_2021.pdf