2026-08-29T09:10:47Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/260552025-12-18T13:01:52Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Marotta, Annalisa author Noro, Fabrizia author Parisi, Roberta author Gialluisi, Alessandro author Tirozzi, Alfonsina author De Curtis, Amalia author Costanzo, Simona author Di Castelnuovo, Augusto author Cerletti, Chiara author Donati, Maria Benedetta author de Gaetano, Giovanni author Iacoviello, Licia author Izzi, Benedetta author Gianfagna, Francesco author 2021-12 Neuromedin U (NMU) is a neuropeptide involved in gut-brain axis, energy balance and immune response. We aimed at analysing the association between epigenetic variability and metabolic indices and the potential mediating role of low-grade inflammation in a general population of Italian adults. Blood DNA methylation levels at two CpG islands ( and ) were analysed using pyrosequencing in a randomly selected sub-cohort of 1,160 subjects from the Moli-sani study (≥35years; 49.20% men). Multivariable regressions adjusted for age, sex, smoking, alcohol and vegetable consumption were performed to estimate the associations between methylation and metabolic phenotypes (BMI, waist-to-hip ratio, blood pressure, glucose, HOMA-IR, lipids, lipoprotein(a) and apolipoproteins). Mediation analysis was performed to identify the influence of low-grade inflammation in the association using a composite index based on C reactive protein, granulocyte-to-lymphocyte ratio (GLR), platelet and white blood cell counts (INFLA-score).Using principal component analysis four methylation factors were identified: and . was FDR significantly associated with total cholesterol (for 1 SD increase: β = 4.5 ± 1.4 mg/dL of, R = 10.8%, p = 0.001), ApoB (0.03 ± 0.01 g/L, 12.2%, p = 0.0004), with INFLA-score (1.05 ± 0.22, = 2.7E-6) and GLR (-0.27 ± 0.03, 30.4%, = 1.3E-20). GLR and lymphocyte numbers mediate the association of with cholesterol (24.0% of total effect, Sobel p = 0.013) and ApoB (42.6%, p = 9E-7), respectively.These findings suggest that promoter methylation patterns could mark a pathway linking lipids with haematopoiesis and systemic inflammation. Epigenetics. 2021 Dec;16(12):1347-1360. Epigenetics 33393847 https://hdl.handle.net/20.500.12105/26055 DNA methylation apolipoprotein B cardiovascular risk granulocyte-to-lymphocyte ratio low-grade inflammation metabolic indices neuromedin U white blood cell count DNA methylation in blood is associated with metabolic and inflammatory indices: results from the Moli-sani study.