2026-06-14T03:39:42Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/260332025-12-18T12:55:45Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19610col_20.500.12105_19617col_20.500.12105_25179
Repisalud
author
González-Caballero, MCarmen
author
De Alba-González, Mercedes
author
Torres-Ruiz, Mónica
author
Iglesias-Hernandez, Patricia
author
Zapata, Verónica
author
Terrón-Orellana, Maria Carmen
author
Sachse, Martin
author
Morales, Mónica
author
Martin-Folgar, Raquel
author
Liste-Noya, Isabel
author
Cañas Portilla, Ana Isabel
funder
Instituto de Salud Carlos III
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2025-01-16T12:22:07Z
2025-01-16T12:22:07Z
2024-05
González-Caballero MC, de Alba González M, Torres-Ruiz M, Iglesias-Hernández P, Zapata V, Terrón MC, Sachse M, Morales M, Martin-Folgar R, Liste I, Cañas-Portilla AI. Internalization and toxicity of polystyrene nanoplastics on inmortalized human neural stem cells. Chemosphere. 2024 May;355:141815.
10.1016/j.chemosphere.2024.141815
1879-1298
0045-6535
Chemosphere
38556182
https://hdl.handle.net/20.500.12105/26033
Global plastic production has increased exponentially in recent decades, and a significant part of it persists in the environment, where it degrades into microplastics and nanoplastics (MPs and NPs). These can enter in humans by ingestion, inhalation, and dermal routes, and there is scientific evidence that they are able to reach the systemic circulation and penetrate and accumulate in various tissues and organs. Neurodevelopmental toxicity of NPs is one of the most worrying effects, as they can cross the blood-brain barrier. In the following study, we analyzed, by transmission electron microscopy, the in vitro uptake of 30-nm polystyrene nanoplastics (PS-NPs) into human neural stem cells (NSCs), their accumulation and subcellular localization within the cell. Furthermore, we studied the effects of different concentrations of PS-NPs on cell death, proliferation, and cell differentiation using immunocytochemistry and quantitative real time PCR for specific markers. This study demonstrated that PS-NPs were able to enter the cell, probably by endocytosis, accumulate, and aggregated in human NSCs, without being detected in the nucleus, causing cell death by apoptosis and decreased cell proliferation. This study provides new insights into the interaction and effects of PS-NPs in human NSC and supports the scientific evidence for the involvement of nanoplastic in neurodevelopmental disorders.
eng
Cell death
Cell proliferation
Human neural stem cells
Nanoplastics
Uptake
Internalization and toxicity of polystyrene nanoplastics on inmortalized human neural stem cells
research article
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URL
https://repisalud.isciii.es/bitstreams/a916856b-5e4a-420f-9b86-91a055efbf7b/download
File
MD5
de2aede3bd202c1bda39c55e3e03255f
19961998
application/pdf
InternalizationToxicityPolystyreneNanoplastics_2024.pdf