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oai:repisalud.isciii.es:20.500.12105/260202025-12-18T13:00:49Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961

00925njm 22002777a 4500 dc Egaña-Gorroño, Lander author Escribà, Tuixent author Boulanger, Nicolas author Guardo, Alberto Crespo author León, Agathe author Bargalló, Manel Enric author García, Felipe author Gatell, José María author Plana, Montserrat author Arnedo, Mireia author HIV Controllers Consortium of the AIDS Spanish Network author Lopez-Galindez, Luis Cecilio author Pernas, Maria author Casado, Concepcion author Alcamí, José author 2014 Background: The emerging relationship between microRNAs (miRNA) and viral-control is a topic of interest in the field of HIV. Host-genome might play an important role in the control of viremia. The aim of this study was to assess the specific miRNA profile that could contribute to the control of HIV replication in Elite Controllers. Results: After adequate normalization, expression profile of 286 human miRNAs (hsa-miR) was evaluated in phytohaemagglutinin-stimulated PBMCs from 29 individuals classified in 4 groups: 8 elite controllers (EC; viral load <50 cp/ml without treatment), 8 viremic progressors (VP; VL>5000 cp/ml without treatment), 8 patients under antiretroviral treatment (ART; VL<200 cp/ml) and 5 uninfected individuals (HIV-) through TaqMan Array Human microRNA Cards v3.0. A differential expression pattern consisting of 23 miRNAs became significantly different when comparing EC and VP. Profiling analysis segregated the population in two different blocks: while EC and HIV- clustered together in the same block (EC/HIV-_block 1), VP and ART individuals clustered together in a second block (VP/ART_block 2). Two inversely expressed miRNA patterns were determined within those two blocks: a set of 4 miRNAs (hsa-miR-221, -27a, -27b and -29b) was up-expressed in EC/HIV-_block and down-expressed in VP/ART_block while 19 miRNAs were down-expressed in block 1 and up-expressed in block 2. Differential miRNAs were successfully validated through individual RT-qPCR assays. Conclusions: Profile in EC resembled HIV- and differentially clusters with VP and ART. Therefore, differential clustering does not rely on undetectable viremia. Egaña-Gorroño L, Escribà T, Boulanger N, Guardo AC, León A, Bargalló ME, Garcia F, Gatell JM, Plana M, Arnedo M; HIV Controllers Consortium of the AIDS Spanish Network. Differential microRNA expression profile between stimulated PBMCs from HIV-1 infected elite controllers and viremic progressors. PLoS One. 2014 Sep 16;9(9):e106360. 10.1371/journal.pone.0106360 1932-6203 PloS one 25225963 https://hdl.handle.net/20.500.12105/26020 Differential microRNA expression profile between stimulated PBMCs from HIV-1 infected elite controllers and viremic progressors.