2026-05-17T01:18:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/260102025-12-18T12:52:08Zcom_20.500.12105_2273com_20.500.12105_2202col_20.500.12105_19571
Repisalud
author
Díaz-Talavera, Alberto
author
Montero-Conde, Cristina
author
Leandro-García, Luis Javier
author
Robledo Batanero, Mercedes
funder
Centro de Investigación Biomédica en Red - CIBERER (Enfermedades Raras)
funder
Instituto de Salud Carlos III
funder
Fundación Banco Santander
funder
Fundación La Caixa
2025-01-13T14:54:35Z
2025-01-13T14:54:35Z
2022-02-03
Biomolecules . 2022 Feb 3;12(2):248.
Biomolecules
35204749
https://hdl.handle.net/20.500.12105/26010
DNA replication can encounter blocking obstacles, leading to replication stress and genome instability. There are several mechanisms for evading this blockade. One mechanism consists of repriming ahead of the obstacles, creating a new starting point; in humans, PrimPol is responsible for carrying out this task. PrimPol is a primase that operates in both the nucleus and mitochondria. In contrast with conventional primases, PrimPol is a DNA primase able to initiate DNA synthesis de novo using deoxynucleotides, discriminating against ribonucleotides. In vitro, PrimPol can act as a DNA primase, elongating primers that PrimPol itself sythesizes, or as translesion synthesis (TLS) DNA polymerase, elongating pre-existing primers across lesions. However, the lack of evidence for PrimPol polymerase activity in vivo suggests that PrimPol only acts as a DNA primase. Here, we provide a comprehensive review of human PrimPol covering its biochemical properties and structure, in vivo function and regulation, and the processes that take place to fill the gap-containing lesion that PrimPol leaves behind. Finally, we explore the available data on human PrimPol expression in different tissues in physiological conditions and its role in cancer.
eng
PrimPol
cancer
genomic instability
polymerase
primase
replication stress
repriming
PrimPol: A Breakthrough among DNA Replication Enzymes and a Potential New Target for Cancer Therapy.
research article
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URL
https://repisalud.isciii.es/bitstreams/b87ef09a-c321-4313-989d-f4552098d22e/download
File
MD5
d34622ff44b9d3cad9af2fa9e2eedd13
6964639
application/pdf
PrimPolABreakthrough-2022.pdf