2026-05-20T04:15:56Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/259682025-12-18T13:00:16Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_25179
Repisalud
author
Cano-Castaño, Beatriz
author
Corral-Lugo, Andres
author
Gato, Eva
author
Terrón-Orellana, Maria Carmen
author
Martin-Galiano, Antonio Javier
author
Sotillo, Javier
author
Perez Gomez, Astrid
author
McConnell, Michael J
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía, Industria y Competitividad (España)
2025-01-09T08:48:36Z
2025-01-09T08:48:36Z
2024-08-27
Cano-Castaño B, Corral-Lugo A, Gato E, Terrón MC, Martín-Galiano AJ, Sotillo J, Pérez A, McConnell MJ. Loss of Lipooligosaccharide Synthesis in Acinetobacter baumannii Produces Changes in Outer Membrane Vesicle Protein Content. Int J Mol Sci. 2024 Aug 27;25(17):9272.
10.3390/ijms25179272
1422-0067
International journal of molecular sciences
39273220
https://hdl.handle.net/20.500.12105/25968
Outer membrane vesicles (OMVs) are nanostructures derived from the outer membrane of Gram-negative bacteria. We previously demonstrated that vaccination with endotoxin-free OMVs isolated from an strain lacking lipooligosaccharide (LOS) biosynthesis, due to a mutation in , provides full protection in a murine sepsis model. The present study characterizes the protein content of highly-purified OMVs isolated from LOS-replete and LOS-deficient strains. Four purification methods were evaluated to obtain highly purified OMV preparations: ultracentrifugation, size exclusion chromatography (SEC), ultracentrifugation followed by SEC, and Optiprep™. OMVs from each method were characterized using nanoparticle tracking analysis and electron microscopy. OMVs from LOS-deficient and LOS-replete strains purified using the Optiprep™ method were subjected to LC-MS/MS analysis to determine protein content. Significant differences in protein composition between OMVs from LOS-deficient and LOS-replete strains were found. Computational analyses using Bepipred 3.0 and SEMA 2.0 indicated that the lack of LOS led to the overexpression of immunogenic proteins found in LOS-containing OMVs and the presence of immune-stimulating proteins absent in LOS-replete OMVs. These findings have important implications for developing OMV-based vaccines against , using both LOS-containing and LOS-free OMVs preparations.
eng
Acinetobacter baumannii
OMVs
Lipooligosaccharide
Outer membrane vesicles
Proteomic
Vaccine
Loss of Lipooligosaccharide Synthesis in Produces Changes in Outer Membrane Vesicle Protein Content
research article
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LossLipooligosaccharideSynthesisAcinetobacter_2024.pdf
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Supplementary_LossLipooligosaccharideSynthesisAcinetobacter_2024.pdf