2026-05-20T04:15:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/259642025-12-18T12:56:58Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19608col_20.500.12105_19609col_20.500.12105_25179
Repisalud
author
Hoefer, Andreas
author
Pampaka, Despina
author
Herrera-León, Silvia
author
Peiró, Sonia
author
Varona Fernandez, Sarai
author
Lopez-Perea, Noemi
author
Masa-Calles, Josefa
author
Herrera-Leon, Laura
2025-01-08T11:12:32Z
2025-01-08T11:12:32Z
2021-02-18
Hoefer A, Pampaka D, Herrera-León S, Peiró S, Varona S, López-Perea N, Masa-Calles J, Herrera-León L. Molecular and Epidemiological Characterization of Toxigenic and Nontoxigenic Corynebacterium diphtheriae, Corynebacterium belfantii, Corynebacterium rouxii, and Corynebacterium ulcerans Isolates Identified in Spain from 2014 to 2019. J Clin Microbiol. 2021 Feb 18;59(3):e02410-20.
10.1128/JCM.02410-20
1098-660X
0095-1137
Journal of clinical microbiology
https://pmc.ncbi.nlm.nih.gov/articles/PMC8106711/
33298610
https://hdl.handle.net/20.500.12105/25964
This study examines the microbiological and epidemiological characteristics of toxigenic and nontoxigenic isolates submitted to the national reference laboratory in Spain, between 2014 and 2019, in order to describe the current situation and improve our knowledge regarding these emerging pathogens. Epidemiological information was extracted from the Spanish Surveillance System. Microbiological and molecular characterization was carried out using phenotypic methods, multilocus sequence typing (MLST), whole-genome sequencing (WGS), and core genome MLST (cgMLST). Thirty-nine isolates were analyzed. Twenty-one isolates were identified as (6 toxigenic), 14 as , 4 as (3 toxigenic), and 1 as One isolate was identified as nontoxigenic gene bearing (NTTB). Ages of patients ranged from 1 to 89 years, with 10% (3/30) of nontoxigenic and 22% (2/9) of toxigenic isolates collected from children less than 15 years. Twenty-five of the patients were males (17/30 in nontoxigenic; 8/9 in toxigenic). MLST identified 28 sequence types (STs), of which 7 were described for the first time in Spain. WGS analysis showed that 10 isolates, including 3 toxigenic isolates, harbored a variety of antibiotic resistance genes in addition to the high prevalence of penicillin resistance phenotypically demonstrated. Phylogenetic analysis revealed one cluster of isolates from family members. Risk information was available for toxigenic isolates (9/39); 3 patients reported recent travels to countries of endemicity and 3 had contact with cats/dogs. One unvaccinated child with respiratory diphtheria had a fatal outcome. Including nontoxigenic infections in disease surveillance and using WGS could further improve current surveillance.
eng
Corynebacterium
Corynebacterium infections
Diphtheria
Diphtheria toxin
Epidemiology
Microbiology
Molecular and Epidemiological Characterization of Toxigenic and Nontoxigenic Corynebacterium diphtheriae, Corynebacterium belfantii, Corynebacterium rouxii, and Corynebacterium ulcerans Isolates Identified in Spain from 2014 to 2019
research article
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