2026-05-20T04:15:54Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/259642025-12-18T12:56:58Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19608col_20.500.12105_19609col_20.500.12105_25179

00925njm 22002777a 4500 dc Hoefer, Andreas author Pampaka, Despina author Herrera-León, Silvia author Peiró, Sonia author Varona Fernandez, Sarai author Lopez-Perea, Noemi author Masa-Calles, Josefa author Herrera-Leon, Laura author 2021-02-18 This study examines the microbiological and epidemiological characteristics of toxigenic and nontoxigenic isolates submitted to the national reference laboratory in Spain, between 2014 and 2019, in order to describe the current situation and improve our knowledge regarding these emerging pathogens. Epidemiological information was extracted from the Spanish Surveillance System. Microbiological and molecular characterization was carried out using phenotypic methods, multilocus sequence typing (MLST), whole-genome sequencing (WGS), and core genome MLST (cgMLST). Thirty-nine isolates were analyzed. Twenty-one isolates were identified as (6 toxigenic), 14 as , 4 as (3 toxigenic), and 1 as One isolate was identified as nontoxigenic gene bearing (NTTB). Ages of patients ranged from 1 to 89 years, with 10% (3/30) of nontoxigenic and 22% (2/9) of toxigenic isolates collected from children less than 15 years. Twenty-five of the patients were males (17/30 in nontoxigenic; 8/9 in toxigenic). MLST identified 28 sequence types (STs), of which 7 were described for the first time in Spain. WGS analysis showed that 10 isolates, including 3 toxigenic isolates, harbored a variety of antibiotic resistance genes in addition to the high prevalence of penicillin resistance phenotypically demonstrated. Phylogenetic analysis revealed one cluster of isolates from family members. Risk information was available for toxigenic isolates (9/39); 3 patients reported recent travels to countries of endemicity and 3 had contact with cats/dogs. One unvaccinated child with respiratory diphtheria had a fatal outcome. Including nontoxigenic infections in disease surveillance and using WGS could further improve current surveillance. Hoefer A, Pampaka D, Herrera-León S, Peiró S, Varona S, López-Perea N, Masa-Calles J, Herrera-León L. Molecular and Epidemiological Characterization of Toxigenic and Nontoxigenic Corynebacterium diphtheriae, Corynebacterium belfantii, Corynebacterium rouxii, and Corynebacterium ulcerans Isolates Identified in Spain from 2014 to 2019. J Clin Microbiol. 2021 Feb 18;59(3):e02410-20. 10.1128/JCM.02410-20 1098-660X 0095-1137 Journal of clinical microbiology https://pmc.ncbi.nlm.nih.gov/articles/PMC8106711/ 33298610 https://hdl.handle.net/20.500.12105/25964 Corynebacterium Corynebacterium infections Diphtheria Diphtheria toxin Epidemiology Microbiology Molecular and Epidemiological Characterization of Toxigenic and Nontoxigenic Corynebacterium diphtheriae, Corynebacterium belfantii, Corynebacterium rouxii, and Corynebacterium ulcerans Isolates Identified in Spain from 2014 to 2019