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oai:repisalud.isciii.es:20.500.12105/259632025-12-18T12:53:12Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Sanchez-Prieto, Sergio author LLorente Rodríguez, Maria Teresa author Ramiro-Jimenez, Raquel author Herrera-Leon, Laura author Herrera-León, Silvia author 2019-07 We evaluated the SHIGA TOXIN QUIK CHEK (STQC) on its suitability for Shiga toxin-producing Escherichia coli (STEC) testing on human fecal samples after overnight enrichment. Our in-house PCR-based protocol for STEC detection was used as the standard for comparison. STQC detected all described Shiga toxin subtypes with the only exception of Stx2f. In comparison to PCR, STQC performed with an overall sensitivity of 55.4%, specificity of 100.0%, positive predictive value of 100.0%, negative predictive value of 73.0%, infinite positive likelihood ratio, and negative likelihood ratio of 0.45. We conclude that STQC may not be considered a suitable screening tool for STEC detection in human fecal samples, although it could be useful for laboratories where PCR is not a routine tool for STEC screening yet, subject to the confirmation of negative samples by a reference laboratory with full diagnostic capabilities. Sánchez S, Llorente MT, Ramiro R, Herrera-León L, Herrera-León S. Evaluation of the SHIGA TOXIN QUIK CHEK after overnight enrichment as screening tool for Shiga toxin-producing Escherichia coli detection in human fecal samples. Diagn Microbiol Infect Dis. 2019 Jul;94(3):218-222. 0732-8893 https://hdl.handle.net/20.500.12105/25963 30885395 10.1016/j.diagmicrobio.2019.01.010 1879-0070 Diagnostic microbiology and infectious disease Evaluation of the SHIGA TOXIN QUIK CHEK after overnight enrichment as screening tool for Shiga toxin-producing Escherichia coli detection in human fecal samples