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oai:repisalud.isciii.es:20.500.12105/259612025-12-18T12:56:00Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Verweij, Frederik J author Balaj, Leonora author Boulanger, Chantal M author Carter, David R F author Compeer, Ewoud B author D'Angelo, Gisela author El Andaloussi, Samir author Goetz, Jacky G author Gross, Julia Christina author Hyenne, Vincent author Krämer-Albers, Eva-Maria author Lai, Charles P author Loyer, Xavier author Marki, Alex author Momma, Stefan author Nolte-'t Hoen, Esther N M author Pegtel, D Michiel author Peinado Selgas, Hector author Raposo, Graça author Rilla, Kirsi author Tahara, Hidetoshi author Théry, Clotilde author van Royen, Martin E author Vandenbroucke, Roosmarijn E author Wehman, Ann M author Witwer, Kenneth author Wu, Zhiwei author Wubbolts, Richard author van Niel, Guillaume author 2021-09 Extracellular vesicles (EVs) are nano-sized lipid bilayer vesicles released by virtually every cell type. EVs have diverse biological activities, ranging from roles in development and homeostasis to cancer progression, which has spurred the development of EVs as disease biomarkers and drug nanovehicles. Owing to the small size of EVs, however, most studies have relied on isolation and biochemical analysis of bulk EVs separated from biofluids. Although informative, these approaches do not capture the dynamics of EV release, biodistribution, and other contributions to pathophysiology. Recent advances in live and high-resolution microscopy techniques, combined with innovative EV labeling strategies and reporter systems, provide new tools to study EVs in vivo in their physiological environment and at the single-vesicle level. Here we critically review the latest advances and challenges in EV imaging, and identify urgent, outstanding questions in our quest to unravel EV biology and therapeutic applications. Nat Methods . 2021 Sep;18(9):1013-1026. Nature Methods https://pmc.ncbi.nlm.nih.gov/articles/PMC8796660/pdf/nihms-1771283.pdf 34446922 https://hdl.handle.net/20.500.12105/25961 TRANSFERRIN RECEPTOR EXOSOME RELEASE HUMAN-DISEASE GAG PROTEIN CELLS SECRETION ELEGANS MICROVESICLES RNA DEGRADATION The power of imaging to understand extracellular vesicle biology in vivo.