2026-08-29T14:17:50Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/259452025-12-18T13:01:22Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Dahdouh, Elias author Gómez-Marcos, Laro author Cañada-Garcia, Javier Enrique author Ramirez de Arellano, Eva author Sánchez-García, Aida author Sanchez-Romero, Isabel author López-Urrutia, Luis author de la Iglesia, Pedro author Gonzalez-Praetorius, Alejandro author Sotelo, Jared author Valle-Millares, Daniel author Alonso-González, Isabela author Bautista, Veronica author Lara Fuella, Noelia author García-Cobos, Silvia author Cercenado, Emilia author Aracil, Belen author Oteo-Iglesias, Jesus author Perez-Vazquez, Maria author Spanish Eco-Carba Study Group author Casquero-García, Verónica author Valiente, Olga author 2024 Introduction: Carbapenemase-Producing Escherichia coli (CP-Eco) isolates, though less prevalent than other CP-Enterobacterales, have the capacity to rapidly disseminate antibiotic resistance genes (ARGs) and cause serious difficult-to-treat infections. The aim of this study is phenotypically and genotypically characterizing CP-Eco isolates collected from Spain to better understand their resistance mechanisms and population structure. Methods: Ninety representative isolates received from 2015 to 2020 from 25 provinces and 59 hospitals Spanish hospitals were included. Antibiotic susceptibility was determined according to EUCAST guidelines and whole-genome sequencing was performed. Antibiotic resistance and virulence-associated genes, phylogeny and population structure, and carbapenemase genes-carrying plasmids were analyzed. Results and discussion: The 90 CP-Eco isolates were highly polyclonal, where the most prevalent was ST131, detected in 14 (15.6%) of the isolates. The carbapenemase genes detected were bla OXA-48 (45.6%), bla VIM-1 (23.3%), bla NDM-1 (7.8%), bla KPC-3 (6.7%), and bla NDM-5 (6.7%). Forty (44.4%) were resistant to 6 or more antibiotic groups and the most active antibiotics were colistin (98.9%), plazomicin (92.2%) and cefiderocol (92.2%). Four of the seven cefiderocol-resistant isolates belonged to ST167 and six harbored bla NDM. Five of the plazomicin-resistant isolates harbored rmt. IncL plasmids were the most frequent (45.7%) and eight of these harbored bla VIM-1. bla OXA-48 was found in IncF plasmids in eight isolates. Metallo-β-lactamases were more frequent in isolates with resistance to six or more antibiotic groups, with their genes often present on the same plasmid/integron. ST131 isolates were associated with sat and pap virulence genes. This study highlights the genetic versatility of CP-Eco and its potential to disseminate ARGs and cause community and nosocomial infections. Dahdouh E, Gómez-Marcos L, Cañada-García JE, de Arellano ER, Sánchez-García A, Sánchez-Romero I, López-Urrutia L, de la Iglesia P, Gonzalez-Praetorius A, Sotelo J, Valle-Millares D, Alonso-González I, Bautista V, Lara N, García-Cobos S, Cercenado E, Aracil B, Oteo-Iglesias J, Pérez-Vázquez M; Spanish Eco-Carba Study Group. Characterizing carbapenemase-producing Escherichia coli isolates from Spain: high genetic heterogeneity and wide geographical spread. Front Cell Infect Microbiol. 2024 May 16;14:1390966. 10.3389/fcimb.2024.1390966 2235-2988 Frontiers in cellular and infection microbiology 38817448 https://hdl.handle.net/20.500.12105/25945 Escherichia coli Antibiotic resistance Carbapenemases Plasmids Sequence type Virulence factor genes Whole-genome sequencing Characterizing carbapenemase-producing isolates from Spain: high genetic heterogeneity and wide geographical spread.