2026-04-29T12:24:17Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/259102025-12-18T12:59:50Zcom_20.500.12105_19586com_20.500.12105_2202col_20.500.12105_19587

00925njm 22002777a 4500 dc Domínguez-Berzosa, Laura author Cantarero, Lara author Rodríguez-Sanz, María author Tort, Gemma author Garrido, Elena author Troya-Balseca, Johanna author Sáez, María author Castro-Martínez, Xóchitl Helga author Fernandez-Lizarbe, Sara author Urquizu, Edurne author Calvo, Enrique author López, Juan Antonio author Palomo, Tomás author Palau, Francesc author Hoenicka, Janet author 2024-10-04 The IA polymorphism is a marker of both the Ankyrin Repeat and Kinase Domain containing I gene () encoding a RIP-kinase, and the gene for the dopamine receptor D2. Despite a large number of studies of IA in addictions and other psychiatric disorders, there is difficulty in interpreting this genetic phenomenon due to the lack of knowledge about ANKK1 function. In SH-SY5Y neuroblastoma models, we show that ANKK1 interacts with the synapse protein FERM ARH/RhoGEF and Pleckstrin Domain 1 (FARP1), which is a guanine nucleotide exchange factor (GEF) of the RhoGTPases RAC1 and RhoA. ANKK1-FARP1 colocalized in F-ACTIN-rich structures for neuronal maturation and migration, and both proteins activate the Wnt/PCP pathway. ANKK1, but not FARP1, promotes neuritogenesis, and both proteins are involved in neuritic spine outgrowth. Notably, the knockdown of or affects RhoGTPases expression and neural differentiation. Additionally, ANKK1 binds WGEF, another GEF of Wnt/PCP, regulating its interaction with RhoA. During neuronal differentiation, ANKK1-WGEF interaction is downregulated, while ANKK1-FARP1 interaction is increased, suggesting that ANKK1 recruits Wnt/PCP components for bidirectional control of F-ACTIN assembly. Our results suggest a brain structural basis in IA-associated phenotypes. Int J Mol Sci. 2024 Oct 4;25(19):10705. International Journal of Molecular Sciences 39409035 https://hdl.handle.net/20.500.12105/25910 ANKK1 F-ACTIN FARP1 TaqIA Wnt/PCP addictions ANKK1 Is a Wnt/PCP Scaffold Protein for Neural F-ACTIN Assembly.