2026-05-17T01:15:58Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/258882025-12-18T12:56:34Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Garcia-Gonzalez, Irene author Gambera, Stefano author Rocha, Susana F author Regano, Alvaro author Garcia-Ortega, Lourdes author Lytvyn, Mariya author Diago-Domingo, Luis author Sanchez-Muñoz, Maria S author Garcia-Cabero, Aroa author Zagorac, Ivana author Luo, Wen author De Andrés-Laguillo, Macarena author Fernández-Chacón, Macarena author Casquero-Garcia, Verónica author Lunella, Federica Francesca author Torroja, Carlos author Sánchez-Cabo, Fátima author Benedito, Rui author 2024-12-13 To understand gene function, it is necessary to compare cells carrying the mutated target gene with normal cells. In most biomedical studies, the cells being compared are in different mutant and control animals and, therefore, do not experience the same epigenetic changes and tissue microenvironment. The experimental induction of genetic mosaics is essential to determine a gene cell-autonomous function and to model the etiology of diseases caused by somatic mutations. Current technologies used to induce genetic mosaics in mice lack either accuracy, throughput or barcoding diversity. Here we present the iFlpMosaics toolkit comprising a large set of new genetic tools and mouse lines that enable recombinase-dependent ratiometric induction and single-cell clonal tracking of multiple fluorescently labeled wild-type and Cre-mutant cells within the same time window and tissue microenvironment. The labeled cells can be profiled by multispectral imaging or by fluorescence-activated flow cytometry and single-cell RNA sequencing. iFlpMosaics facilitate the induction and analysis of genetic mosaics in any quiescent or progenitor cell, and for any given single or combination of floxed genes, thus enabling a more accurate understanding of how induced genetic mutations affect the biology of single cells during tissue development, homeostasis and disease. Nat Methods. 2024 Dec 13. Nature Methods 39672980 https://hdl.handle.net/20.500.12105/25888 iFlpMosaics enable the multispectral barcoding and high-throughput comparative analysis of mutant and wild-type cells.