2026-05-20T04:15:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/255732025-12-18T13:01:31Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_25179
Repisalud
author
Tajuelo, Ana
author
Gato, Eva
author
Oteo-Iglesias, Jesus
author
Perez-Vazquez, Maria
author
McConnell, Michael J
author
Martin-Galiano, Antonio Javier
author
Perez Gomez, Astrid
author
McConnell, Michael J
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
National University of Distance Education (España)
2024-11-21T17:17:04Z
2024-11-21T17:17:04Z
2024-09-11
Int J Mol Sci. 2024 Sep 11;25(18):9837.
10.3390/ijms25189837
1422-0067
Molecular Diversity Preservation International
39337325
https://hdl.handle.net/20.500.12105/25573
Despite its medical relevance, there is no commercial vaccine that protects the population at risk from multidrug-resistant (MDR) infections. The availability of massive omic data and novel algorithms may improve antigen selection to develop effective prophylactic strategies. Up to 133 exposed proteins in the core proteomes, between 516 and 8666 genome samples, of the six most relevant MDR clonal groups (CGs) carried conserved B-cell epitopes, suggesting minimized future evasion if utilized for vaccination. Antigens showed a range of epitopicity, functional constraints, and potential side effects. Eleven antigens, including three sugar porins, were represented in all MDR-CGs, constitutively expressed, and showed limited reactivity with gut microbiota. Some of these antigens had important interactomic interactions and may elicit adhesion-neutralizing antibodies. Synergistic bivalent to pentavalent combinations that address expression conditions, interactome location, virulence activities, and clone-specific proteins may overcome the limiting protection of univalent vaccines. The combination of five central antigens accounted for 41% of all non-redundant interacting partners of the antigen dataset. Specific antigen mixtures represented in a few or just one MDR-CG further reduced the chance of microbiota interference. Rational antigen selection schemes facilitate the design of high-coverage and "magic bullet" multivalent vaccines against recalcitrant lineages.
eng
Antibiotic resistance
Betweenness centrality
Epitope
Immunoinformatics
Intrahospital infection
Natural processing language
Neutralizing antibody
Protein hub
Reverse vaccinology
Virulence factor
Deep Intraclonal Analysis for the Development of Vaccines against Drug-Resistant Lineages
research article
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DeepIntraclonalAnalysisDevelopment_2024.pdf
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Table_S1_List_antigens_with_conserved_Bcell_epitopic_zones_in_Kpneumoniae_MDR-CGs_BK2024_06_13.xlsx
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Table_S2_List_of_MDR-CG_conserved_Bcell_epitopes.xlsx
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Table_S3_Prediction_of_applicability_of_antigens_for_univalent_Kpneumoniae_vaccines.xlsx
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Table_S4_Representative_Kpneumoniae_ST_genomes.xlsx