2026-05-20T04:15:56Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/255732025-12-18T13:01:31Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_25179

00925njm 22002777a 4500 dc Tajuelo, Ana author Gato, Eva author Oteo-Iglesias, Jesus author Perez-Vazquez, Maria author McConnell, Michael J author Martin-Galiano, Antonio Javier author Perez Gomez, Astrid author McConnell, Michael J author 2024-09-11 Despite its medical relevance, there is no commercial vaccine that protects the population at risk from multidrug-resistant (MDR) infections. The availability of massive omic data and novel algorithms may improve antigen selection to develop effective prophylactic strategies. Up to 133 exposed proteins in the core proteomes, between 516 and 8666 genome samples, of the six most relevant MDR clonal groups (CGs) carried conserved B-cell epitopes, suggesting minimized future evasion if utilized for vaccination. Antigens showed a range of epitopicity, functional constraints, and potential side effects. Eleven antigens, including three sugar porins, were represented in all MDR-CGs, constitutively expressed, and showed limited reactivity with gut microbiota. Some of these antigens had important interactomic interactions and may elicit adhesion-neutralizing antibodies. Synergistic bivalent to pentavalent combinations that address expression conditions, interactome location, virulence activities, and clone-specific proteins may overcome the limiting protection of univalent vaccines. The combination of five central antigens accounted for 41% of all non-redundant interacting partners of the antigen dataset. Specific antigen mixtures represented in a few or just one MDR-CG further reduced the chance of microbiota interference. Rational antigen selection schemes facilitate the design of high-coverage and "magic bullet" multivalent vaccines against recalcitrant lineages. Int J Mol Sci. 2024 Sep 11;25(18):9837. 10.3390/ijms25189837 1422-0067 Molecular Diversity Preservation International 39337325 https://hdl.handle.net/20.500.12105/25573 Antibiotic resistance Betweenness centrality Epitope Immunoinformatics Intrahospital infection Natural processing language Neutralizing antibody Protein hub Reverse vaccinology Virulence factor Deep Intraclonal Analysis for the Development of Vaccines against Drug-Resistant Lineages