2026-05-23T01:08:32Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/255302025-12-18T12:59:37Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16966col_20.500.12105_16973col_20.500.12105_19617
Repisalud
author
Medel-Martínez, Ana
author
Redrado-Osta, Ana
author
Crespo-Barreda, Alejandra
author
Sancho-Albero, Maria
author
Sánchez, Lourdes
author
Sebastián, Víctor
author
Pardo, María
author
De la Vieja, Antonio
author
Martin-Duque, Pilar
funder
Instituto de Salud Carlos III
funder
Asociación de Padres de Niños Oncológicos de Aragón
funder
Asociación Española Contra el Cáncer
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación Ramón Areces
2024-11-19T11:52:19Z
2024-11-19T11:52:19Z
2024-07-08
Biomolecules. 2024 Jul 8;14(7):810.
10.3390/biom14070810
2218-273X
Biomolecules
39062527
https://hdl.handle.net/20.500.12105/25530
Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk-exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population.
eng
Casein
Characterization
Extraction protocol
Milk exosomes
Milk fat globules
Rennet
Storage
Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
research article
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URL
https://repisalud.isciii.es/bitstreams/2c2a2adc-8ea5-47fc-8a41-62b09fad3523/download
File
MD5
f5dd895f2d8200dff9d8e0e58d4b35c8
3797465
application/pdf
IsolationCharacterizationMilkExosomes_2024.pdf