2026-05-22T23:07:53Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/255302025-12-18T12:59:37Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16966col_20.500.12105_16973col_20.500.12105_19617

00925njm 22002777a 4500 dc Medel-Martínez, Ana author Redrado-Osta, Ana author Crespo-Barreda, Alejandra author Sancho-Albero, Maria author Sánchez, Lourdes author Sebastián, Víctor author Pardo, María author De la Vieja, Antonio author Martin-Duque, Pilar author 2024-07-08 Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk-exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population. Biomolecules. 2024 Jul 8;14(7):810. 10.3390/biom14070810 2218-273X Biomolecules 39062527 https://hdl.handle.net/20.500.12105/25530 Casein Characterization Extraction protocol Milk exosomes Milk fat globules Rennet Storage Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies