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oai:repisalud.isciii.es:20.500.12105/254992025-12-18T13:01:18Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16972

00925njm 22002777a 4500 dc Fernández-Arévalo, Anna author Gonzalez, Estela author Ballart, Cristina author Martin-Martin, Ines author Tebar, Silvia author Muñoz, Carme author Jimenez, Maribel author Molina, Ricardo author Gállego, Montserrat author 2024-02 In 2009, a large outbreak of leishmaniasis, associated with environmental changes, was declared near Madrid (Spain), in which Phlebotomus perniciosus was the vector, whereas the main reservoirs were hares and rabbits. Analysis of isolates from humans, vectors and leporids from the focus identified the Leishmania infantum ITS-Lombardi genotype. However, multilocus enzyme electrophoresis (MLEE), the reference technique for Leishmania typing, and sequencing of the hsp70 gene, a commonly used marker, were not performed. In the present study, 19 isolates from P. perniciosus (n = 11), hares (n = 5) and rabbits (n = 3) from the outbreak area, all characterized as ITS-Lombardi in previous studies, were analysed by MLEE and hsp70 sequencing. The hsp70 results confirmed that all the analysed strains are L. infantum. However, by MLEE, 4 different zymodemes of L. infantum were identified based on variable mobilities of the NP1 enzyme: MON-34 (NP1100, n = 11), MON-80 (NP1130, n = 6), MON-24 (NP1140, n = 1) and MON-331 (NP1150, n = 1). The relative frequency of these zymodemes does not correspond to their usual occurrence in Spain. Moreover, MON-34 and MON-80 were found in P. perniciosus, hares and rabbits for the first time. These findings continue to provide insights into the outbreak and call for further studies with a higher number of strains. Parasitology. 2024 Feb;151(2):213-219. 0031-1820 https://hdl.handle.net/20.500.12105/25499 38105582 10.1017/S0031182023001336 1469-8161 Parasitology Leishmania Spain Outbreak Typing Typing of isolates from vectors and leporids of the Madrid (Spain) outbreak